Protein Info for PP_0725 in Pseudomonas putida KT2440

Annotation: conserved hypothetical TPR repeat protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 573 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF13432: TPR_16" amino acids 222 to 271 (50 residues), 15.6 bits, see alignment 1.5e-05 amino acids 259 to 317 (59 residues), 32.6 bits, see alignment 7.5e-11 amino acids 301 to 345 (45 residues), 18.7 bits, see alignment 1.7e-06 amino acids 357 to 421 (65 residues), 17.7 bits, see alignment E=3.5e-06 amino acids 426 to 491 (66 residues), 18.7 bits, see alignment E=1.7e-06 amino acids 497 to 545 (49 residues), 20.4 bits, see alignment 5e-07 PF14559: TPR_19" amino acids 235 to 292 (58 residues), 36.1 bits, see alignment 5.8e-12 PF13174: TPR_6" amino acids 396 to 420 (25 residues), 16.2 bits, see alignment (E = 1.1e-05) amino acids 497 to 524 (28 residues), 13.8 bits, see alignment (E = 6.6e-05) PF13374: TPR_10" amino acids 494 to 519 (26 residues), 15.2 bits, see alignment (E = 1.5e-05) PF13181: TPR_8" amino acids 496 to 522 (27 residues), 12.3 bits, see alignment (E = 0.00014)

Best Hits

Swiss-Prot: 67% identical to Y4667_PSEAE: TPR repeat-containing protein PA4667 (PA4667) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0725)

Predicted SEED Role

"FIG140336: TPR domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PX3 at UniProt or InterPro

Protein Sequence (573 amino acids)

>PP_0725 conserved hypothetical TPR repeat protein (Pseudomonas putida KT2440)
MNKPYALLLAFALLQGCQSLAPQKAEPPVAEAGKGEAEKPVVYGSFKQDTLYSLLVAELA
GQRNRFDIALANYTDQAAKTQDPAVSERAYRIAEYLGADEPSLENALVWARNDPQNLDAQ
RAAAIQLARAGRYDESMTYMEKVLQGQGDTHFDFLALSAAETDQSTRDGLLHSFERLLVK
YPDNGQLIFGKALLLNQDGKTEEALELLESHPAQNGEVAPILLRARLLQALDRGPEALPL
LRGAIRDNPDDKRLRLTYARTLVEQDRIADAKGEFISLVQQYPDDDELRYSLALVCLENK
DWDEAEGYLQELVERDSYVDAAHLNLGRIHEERHDPAGALREYALVGPGPDYLPAQLRQA
DILIANGRGTEASRLLADAREAQPDYAIQLFLIESESYSNNNKDAQANQVLQQAIQRYPD
DLNLLYTRAMLAEKRNDLTQMEKDLRAIITREPENAMALNALGYTLADRTTRYTEAKALI
DKAHQLTPDDPAVLDSLGWVNYRLGNLDAAETYLRQAFANFPDHEVAAHLGEVLWANGKR
REARQVWAKGFEAQADSPILRKTLLRLTGSETL