Protein Info for PP_0713 in Pseudomonas putida KT2440

Annotation: Kef-type potassium/proton antiporter, CPA2 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 32 to 51 (20 residues), see Phobius details amino acids 57 to 76 (20 residues), see Phobius details amino acids 88 to 111 (24 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 150 to 172 (23 residues), see Phobius details amino acids 184 to 204 (21 residues), see Phobius details amino acids 216 to 234 (19 residues), see Phobius details amino acids 240 to 258 (19 residues), see Phobius details amino acids 270 to 290 (21 residues), see Phobius details amino acids 296 to 315 (20 residues), see Phobius details amino acids 327 to 349 (23 residues), see Phobius details amino acids 361 to 381 (21 residues), see Phobius details TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 15 to 286 (272 residues), 270 bits, see alignment E=1.3e-84 PF00999: Na_H_Exchanger" amino acids 17 to 376 (360 residues), 212.6 bits, see alignment E=4.3e-67

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0713)

Predicted SEED Role

"Glutathione-regulated potassium-efflux system protein KefB" in subsystem Glutathione-regulated potassium-efflux system and associated functions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PY5 at UniProt or InterPro

Protein Sequence (398 amino acids)

>PP_0713 Kef-type potassium/proton antiporter, CPA2 family (Pseudomonas putida KT2440)
MPHDGSLLQATVVFLLAVVLLVPLAQRLKMGAVPGYLLAGILIGPSVFGLLGNPDNVARL
SEMGVVMLLFVIGLELSPKRLWTMRRALFGVGSLQVGLTAVLLGLLAFWLFDQSKPAAIV
LGLGLALSSTAFGLQVLAERKDLGKPHGRMAVAILLFQDIAAIPLIAVVPLLGGDVSTAD
EGAWPLLAVVVGIGVLIVFGRYLLTPIFKWTVGSGLPELSTATALLVVLGTAWLMEHVGV
SMALGAFLAGVLMAESPFRHELETQIEPIKGLFLGLFFVGVGMSADLRLLFGMPLMVLGL
TLLLVAIKLPLLYGLGRTMGGLNKSQALCLGVVLASGGEFAFVVFKLALEHQVLTQAVHD
LLVLAITLSMAVVPLVMMALARQLHDDSPVQASPETGQ