Protein Info for PP_0624 in Pseudomonas putida KT2440

Annotation: Uncharacterized protein YfiH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02578: Cu-oxidase_4
  • amino acids 24 to 242 (219 residues), 251 bits, E=5.3e-79
• TIGR00726: YfiH family protein
  • amino acids 29 to 243 (215 residues), 199.1 bits, E=3.2e-63

Best Hits

Swiss-Prot: 70% identical to POLOX_PSEAE: Polyphenol oxidase (PA4543) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K05810, conserved hypothetical protein (inferred from 100% identity to ppu:PP_0624)

MetaCyc: 56% identical to purine nucleoside phosphorylase YfiH (Escherichia coli K-12 substr. MG1655)
Adenosine deaminase. [EC: 3.5.4.4]; S-methyl-5'-thioadenosine phosphorylase. [EC: 3.5.4.4, 2.4.2.28]; Purine-nucleoside phosphorylase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; 2.4.2.1 [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; Laccase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1, 1.10.3.2]

Predicted SEED Role

"COG1496: Uncharacterized conserved protein"

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• ureide biosynthesis (6/7 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• purine ribonucleosides degradation (5/6 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• S-methyl-5'-thioadenosine degradation II (1/1 steps found)
• adenine and adenosine salvage V (2/3 steps found)
• superpathway of guanine and guanosine salvage (2/3 steps found)
• superpathway of purine deoxyribonucleosides degradation (4/7 steps found)
• 5'-deoxyadenosine degradation I (1/3 steps found)
• nucleoside and nucleotide degradation (halobacteria) (2/6 steps found)
• L-methionine salvage cycle III (5/11 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• sesamin biosynthesis (1/8 steps found)
• nucleoside and nucleotide degradation (archaea) (2/10 steps found)
• justicidin B biosynthesis (1/10 steps found)
• matairesinol biosynthesis (1/10 steps found)
• salinosporamide A biosynthesis (4/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Methionine metabolism
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1, 3.5.4.4

Use Curated BLAST to search for 1.10.3.2 or 2.4.2.1 or 2.4.2.28 or 3.5.4.4

Sequence Analysis Tools

See Q88Q72 at UniProt or InterPro

Protein Sequence (246 amino acids)

>PP_0624 Uncharacterized protein YfiH (Pseudomonas putida KT2440)
MSDLTQALLFPDWPAPASVRACVTTRQGGVSLPPYETFNLGDHVGDDPAAVAENRRRLSE
QFTIQPAWLKQVHGRVVADADPTVVAEADASWTRQPGIACTVMTADCLPALFCDRAGTRV
AAAHAGWRGLAGGVLEATLDRLALPPEEVLVWLGPAIGPQAFEVGLEVRDAFTAVHPQAA
RAFVDGERPGKLLADIYELARIRLAARGVTAVYGGGLCTVSDARFFSYRRTPQGGRFASL
VWLDPR