Protein Info for PP_0561 in Pseudomonas putida KT2440

Annotation: thiol/disulfide interchange protein 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 590 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 172 to 198 (27 residues), see Phobius details amino acids 211 to 235 (25 residues), see Phobius details amino acids 247 to 270 (24 residues), see Phobius details amino acids 291 to 321 (31 residues), see Phobius details amino acids 334 to 356 (23 residues), see Phobius details amino acids 368 to 386 (19 residues), see Phobius details amino acids 392 to 413 (22 residues), see Phobius details amino acids 420 to 440 (21 residues), see Phobius details PF11412: DsbD_N" amino acids 39 to 148 (110 residues), 106 bits, see alignment E=4e-34 PF02683: DsbD_TM" amino acids 169 to 386 (218 residues), 250.1 bits, see alignment E=4.8e-78 PF13386: DsbD_2" amino acids 175 to 375 (201 residues), 35.6 bits, see alignment E=2.7e-12 PF13899: Thioredoxin_7" amino acids 477 to 558 (82 residues), 63.8 bits, see alignment E=3.8e-21 PF00085: Thioredoxin" amino acids 477 to 580 (104 residues), 29.9 bits, see alignment E=1.4e-10 PF13098: Thioredoxin_2" amino acids 485 to 581 (97 residues), 38.7 bits, see alignment E=3.3e-13

Best Hits

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 100% identity to ppu:PP_0561)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QD3 at UniProt or InterPro

Protein Sequence (590 amino acids)

>PP_0561 thiol/disulfide interchange protein 1 (Pseudomonas putida KT2440)
MRRLFFMLFLLLASPAFAAGLLDNRPSATLGAASLANNADFLPVHEAFKLNLVQADAQTI
KLRFVATDGYYLYRHRFQFRTEPADITLGTPNIPKGEAKHDEFFGDVEVYHGVLDIELPR
KDPRAFTLLVGYQGCADKGLCYPPETARLSIDGEGVANTPATREHGWNWKSLLLFFLAGV
GLTFTPCVLPMLPILSGVVLRGQVGGVRGLALSLAYVLPMAASFAVLGALMGLFGASLNL
QARLQSAWVLVPFALFFVVFALAMFGLFELRLPQALSNRLNNVANHTRGGSLLGAAVLGV
LSSLLVSPCVSAPLAGALLYISASGDALGGALKLFALGLGMGAPLLLVATGGAAWLPKSG
PWMNTVKNAIGVLLLGLAIGLLSRVLPGPATLLLVGFLAAGVALFLGALEFVVKTTAQRL
AQLVGLALLVYALACWYGALTGQGDPLRPLPGASLAASGSLPAANADTWQTITTPDALDA
ALAQAQATGQPVLLDWYADWCISCKVIEHEVLNAPQVQTQLAGFKLLRFDITESNAEQRK
LLDRYQLFGPPALLFFAANGSEITADRVIGEINAGEFAQILTRVRGKVGL