Protein Info for PP_0550 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 214 PF13460: NAD_binding_10" amino acids 12 to 155 (144 residues), 31.8 bits, see alignment E=6.4e-12

Best Hits

Swiss-Prot: 50% identical to YRAR_ECOLI: Putative NAD(P)-binding protein YraR (yraR) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to ppf:Pput_0589)

Predicted SEED Role

"Nucleoside-diphosphate-sugar epimerases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QE4 at UniProt or InterPro

Protein Sequence (214 amino acids)

>PP_0550 conserved protein of unknown function (Pseudomonas putida KT2440)
MYLTPQHVLLAGATGLTGEHLLDRLLNEPTITRVLAPTRRPLAEHPHLENPVGDPAVFLP
QLAGRVDIAYCCLGTTLKQAGSESAFRAVDLDMVVAFSKRAREMGARHLLVISAVGADPK
SSIFYNRVKGEMEEALKAQDWPQLTIVRPSFLLGERLEPRLTEQLVAPFSRFIPGKYRGI
EACTLARALWRLALEEEDGVRVVESDELRRLGRI