Protein Info for PP_0504 in Pseudomonas putida KT2440

Annotation: Outer membrane protein OprG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 227 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13505: OMP_b-brl" amino acids 9 to 227 (219 residues), 49.7 bits, see alignment E=7.7e-17 PF03922: OmpW" amino acids 24 to 227 (204 residues), 252.2 bits, see alignment E=5.8e-79 PF02462: Opacity" amino acids 119 to 191 (73 residues), 21.2 bits, see alignment E=3.8e-08

Best Hits

Swiss-Prot: 46% identical to OMPW_VIBCH: Outer membrane protein W (ompW) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K07275, outer membrane protein (inferred from 100% identity to ppu:PP_0504)

Predicted SEED Role

"Outer membrane protein W precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QI8 at UniProt or InterPro

Protein Sequence (227 amino acids)

>PP_0504 Outer membrane protein OprG (Pseudomonas putida KT2440)
MNKSLLGASLVALALAAPAAHAYQAGDMILRAGAITTAPNESSGDLKFDGNKVSGTKATL
DSDTQLGLTFAYMLTDHIGLELLAATPFKHTVGVKGLGGGLDGKLADIKQLPPTLSLQYY
PMEPNSRFQPYAGVGINYTLFFDEDLSSARKEQGFSNLKLQDSVGIAGQLGMDYMLTDNL
LVNASVWYVDIDTKASVNGPTALGYSKTKVDVDVDPWVYMVGLGYKF