Protein Info for PP_0496 in Pseudomonas putida KT2440

Annotation: Sodium/alanine symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 60 to 83 (24 residues), see Phobius details amino acids 89 to 110 (22 residues), see Phobius details amino acids 139 to 159 (21 residues), see Phobius details amino acids 176 to 194 (19 residues), see Phobius details amino acids 203 to 222 (20 residues), see Phobius details amino acids 235 to 256 (22 residues), see Phobius details amino acids 291 to 315 (25 residues), see Phobius details amino acids 340 to 361 (22 residues), see Phobius details amino acids 374 to 392 (19 residues), see Phobius details amino acids 398 to 421 (24 residues), see Phobius details TIGR00835: amino acid carrier protein" amino acids 14 to 431 (418 residues), 466 bits, see alignment E=5.6e-144 PF01235: Na_Ala_symp" amino acids 49 to 444 (396 residues), 500.8 bits, see alignment E=1.8e-154

Best Hits

Swiss-Prot: 46% identical to ALST_BACSU: Amino-acid carrier protein AlsT (alsT) from Bacillus subtilis (strain 168)

KEGG orthology group: K03310, alanine or glycine:cation symporter, AGCS family (inferred from 100% identity to ppf:Pput_0530)

Predicted SEED Role

"Probable AGCS sodium/alanine/glycine symporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QJ5 at UniProt or InterPro

Protein Sequence (480 amino acids)

>PP_0496 Sodium/alanine symporter (Pseudomonas putida KT2440)
MLEVINDFLSGKLLIVLIVGLGSYFTIRSRFVQFRHFTHMFSVFKESLRGQAGQLSSFQA
LMLSLAGRVGAGNIAGVGIAVTLGGPGAVFWMWVTALVGMSSSFFECTLAQVYKRADGDG
LYRGGPAYYIQHGLKRKSMAIVFSILLLVTYGFAFIGLQSYTVTHSLQNAFAFDPSHTGI
VLAVLLAITFIGGIKRIAAVSDLLVPVKTLAYIGVTLYVIGTQIEHVPAMLETIFKSAFG
LDPAFAGLLGSAIVMGVKRGVFANEAGLGSAPNVAAVAAVKHPGAQGVVQAFSVFLDTFV
ICTCTALLILLSGFYTPGFEGDGIVLTQNSLAAVVGDWGRVFVSVALSLFVFTCILYNYY
LGENSLQFLSRNRVVLMVFRGLVLALVVWGSMQDLSTVFAFADITMTCLAFVNLVALALL
FKVGLRVMRDYDDQRKAGVKQPVFDSSKFADLDLDRNAWPANPQVETATDKAVGQVQTQR