Protein Info for PP_0466 in Pseudomonas putida KT2440

Annotation: 50S ribosomal protein L5

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 179 PF00281: Ribosomal_L5" amino acids 24 to 80 (57 residues), 94 bits, see alignment E=5.3e-31 PF00673: Ribosomal_L5_C" amino acids 84 to 177 (94 residues), 136.2 bits, see alignment E=3.4e-44

Best Hits

Swiss-Prot: 100% identical to RL5_PSEPG: 50S ribosomal protein L5 (rplE) from Pseudomonas putida (strain GB-1)

KEGG orthology group: K02931, large subunit ribosomal protein L5 (inferred from 99% identity to ppw:PputW619_4737)

MetaCyc: 68% identical to 50S ribosomal subunit protein L5 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"LSU ribosomal protein L5p (L11e)" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QM3 at UniProt or InterPro

Protein Sequence (179 amino acids)

>PP_0466 50S ribosomal protein L5 (Pseudomonas putida KT2440)
MARLKEIYRNEIAPKLKEELKLSNVMEVPRVTKITLNMGLGEAIGDKKVIEHAVADLEKI
TGQKPVVTFARKSIAGFKVREGWPIGVKVTLRSDKMYEFLDRLLAISLPRVRDFRGLNAK
SFDGRGNYSMGVKEQIIFPEIDYDKIDALRGLDITLTTTARSDDEGRALLRAFKFPFRN