Protein Info for PP_0396 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 PF04285: DUF444" amino acids 4 to 419 (416 residues), 610.8 bits, see alignment E=7.4e-188

Best Hits

Swiss-Prot: 100% identical to Y430_PSEP1: UPF0229 protein Pput_0430 (Pput_0430) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K09786, hypothetical protein (inferred from 100% identity to ppf:Pput_0430)

Predicted SEED Role

"FIG002076: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P59351 at UniProt or InterPro

Protein Sequence (423 amino acids)

>PP_0396 conserved protein of unknown function (Pseudomonas putida KT2440)
MSYVIDRRLNGKNKSTVNRQRFLRRYREHIKKAVEEAVSRRSIMDMEHGEQISIPGRDID
EPVLHHGRGGKQTIVHPGNKEFTAGEHIPRPQGGGGGGGRGKAGNSGEGMDEFVFQITQE
EFLEFMFEDLELPNLVKRHLTGADTFKTVRAGIANEGNPSRINIVRTLRSAHARRIALTG
SSRALLREAQKELDRLRVEEPDNFTDIQEVEQEIERLKARINRLPFLDTFDLKYNLLVKQ
PNPSSKAVMFCLMDVSGSMTQATKDIAKRFFILLYLFLKRNYERIEVVFIRHHTSAREVD
EEEFFYSRETGGTIVSSALKLMQEIMAERYPASDWNIYAAQASDGDNWNDDSPICRDILS
KQIMPHVQYYTYVEITPREHQALWYEYERIGDAFPDTFAQQQLVSAGDIYPVFRELFQRR
LAT