Protein Info for PP_0393 in Pseudomonas putida KT2440

Annotation: putative 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01498: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
  • amino acids 6 to 130 (125 residues), 65.4 bits, E=2.8e-22
• PF01288: HPPK
  • amino acids 7 to 130 (124 residues), 76.1 bits, E=1.5e-25

Best Hits

KEGG orthology group: K00950, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase [EC: 2.7.6.3] (inferred from 100% identity to ppu:PP_0393)

Predicted SEED Role

"2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3)" in subsystem Folate Biosynthesis (EC 2.7.6.3)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis IV (Plasmodium) (2/3 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I (3/5 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis V (Pyrococcus) (1/4 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis II (Methanocaldococcus) (2/7 steps found)
• tetrahydromethanopterin biosynthesis (3/14 steps found)

KEGG Metabolic Maps

• Folate biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.3

Use Curated BLAST to search for 2.7.6.3

Sequence Analysis Tools

See Q88QU3 at UniProt or InterPro

Protein Sequence (169 amino acids)

>PP_0393 putative 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (Pseudomonas putida KT2440)
MSLSTVYLGLGSNIDREAHLCAGLDALAGILTDMRCSPAFESQAVGIKSGPFINFVVTGQ
TALPLIELDRRLKFIEADNGRYAPDRKGLPLDIDVLMYDDLHGTFDGLVLPRAEILKNAF
VLWPLSLLAPDLVHPGVGKCMAQLWQEAQIDQVLAPVAFEWRGLQLTPA