Protein Info for PP_0390 in Pseudomonas putida KT2440

Annotation: tRNA N6-adenosine threonylcarbamoyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 3 to 317 (315 residues), 442.9 bits, see alignment E=6e-137 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 4 to 310 (307 residues), 402.4 bits, see alignment E=1.3e-124 PF00814: TsaD" amino acids 24 to 311 (288 residues), 330.2 bits, see alignment E=1.2e-102 PF22521: HypF_C_2" amino acids 232 to 306 (75 residues), 29.6 bits, see alignment E=6.3e-11

Best Hits

Swiss-Prot: 100% identical to TSAD_PSEPK: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 100% identity to ppu:PP_0390)

MetaCyc: 71% identical to N6-L-threonylcarbamoyladenine synthase, TsaD subunit (Escherichia coli K-12 substr. MG1655)
RXN-14570 [EC: 2.3.1.234]

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.234 or 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QU6 at UniProt or InterPro

Protein Sequence (341 amino acids)

>PP_0390 tRNA N6-adenosine threonylcarbamoyltransferase (Pseudomonas putida KT2440)
MLVLGLETSCDETGVALYDSERGLLADALFSQIDLHRVFGGVVPELASRDHVKRMLPLIR
QVLDEAGCVATEIDAIAYTAGPGLVGALLVGASCAQALAFAWDIPAIGVHHMEGHLLAPM
LEENPPEFPFVALLVSGGHTQLVRVDGIGQYELLGESLDDAAGEAFDKTAKLIGLNYPGG
PEIARLAEQGVPGRFVFPRPMTDRPGLEFSFSGLKTFALNTWQQCKNAGDDSEQTRCDLS
LAFQQAVVETLTIKCKRALKQTGLKRLVIAGGVSANKALRASLEDMLGSIKGNVYYARPQ
FCTDNGAMIAYAGCQRLLAGQQQDLAISVQARWPMEQLPPL