Protein Info for PP_0340 in Pseudomonas putida KT2440

Annotation: Glutamate-ammonia-ligase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 977 PF03710: GlnE" amino acids 45 to 294 (250 residues), 291.4 bits, see alignment E=5.6e-91 amino acids 572 to 824 (253 residues), 309.1 bits, see alignment E=2.2e-96 PF08335: GlnD_UR_UTase" amino acids 314 to 452 (139 residues), 106.2 bits, see alignment E=1.5e-34 amino acids 847 to 945 (99 residues), 31.1 bits, see alignment E=2.2e-11

Best Hits

Swiss-Prot: 100% identical to GLNE_PSEPK: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to ppu:PP_0340)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QZ4 at UniProt or InterPro

Protein Sequence (977 amino acids)

>PP_0340 Glutamate-ammonia-ligase adenylyltransferase (Pseudomonas putida KT2440)
MRLPLPSDLPATLQPLVTRNQQFISDAVAGHPELDLQAWSPLHRQQFDQVAAASEFVLSL
AQREPAMLFALLASGELERRYAPGELRGQIAATAQAAQSEDELARNLRRARNRQQLRIIW
RDITRQAELGETCRDLSDLADAAIDEAYQWLYPRHCQQFGTPIGNRSGQPQHMVVLGMGK
LGAVELNLSSDIDLIFGFPEGGETEGVKRSLDNQEFFTRLGQRLIKALDPVTVDGFVFRV
DMRLRPYGSAGALVLSFNALEQYYQDQGRDWERYAMIKARVVAGDQAAGAQLQEMLRPFV
YRRYLDFSAIEALRTMKQLIQQEVRRKGMAENIKLGAGGIREVEFIAQAFQLIHGGRDLS
LQQRPLLKVLATLEGQGYLPPAVVAELREGYEFLRYTEHAIQAIADRQTQMLPEGETDQA
RVAYVLGFADWQSFHDQLMYWRGRIDWHFRQVIADPDDEDGEGELVVGGEWSPLWEQAQD
EEAAGRQLQEAGFKQPAEALRRLAGLRSSPQLRSMQRIGRERLDAFIPRLLAQAVEHDNP
DLVLERVLPLVEAVARRSAYLVLLTENPGALRRLLTLCAASPWIAEQIALYPLLLDELLN
EGRLFSPPLAPELASELRERLTRIPEDDLEQQMEALRHFKLAHSLRVAASEISGNLPLMK
VSDYLTWLAEAILDQVLALAWRQTVARHGQPKRSDGSLCDPGFIIIGYGKMGGLELGHGS
DLDLVFIHDGDPQAETDGAKPIDSAQFFTRLGQRIIHLLTTQTNSGQLYDVDMRLRPSGA
SGLLVSSLGAFERYQQNEAWTWEHQALVRARVLVGCKQVGAAFEGVRAKVLGQARDLEKL
RGEVSEMRAKMRDNLGTKATAAGTAANAFDAGVPFDIKQDAGGIVDIEFMVQYAALAWSH
DHPAILRWTDNIRILEELEQANLMPASDAVLLREVYKAFRSASHRQALQKEAGVIDAAQF
ADERREVRRIWGELGLS