Protein Info for PP_0325 in Pseudomonas putida KT2440

Annotation: sarcosine oxidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1004 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 2 to 1004 (1003 residues), 1548.3 bits, see alignment E=0 PF13510: Fer2_4" amino acids 17 to 102 (86 residues), 77.4 bits, see alignment E=3.9e-25 PF07992: Pyr_redox_2" amino acids 172 to 485 (314 residues), 50.2 bits, see alignment E=1.4e-16 PF00890: FAD_binding_2" amino acids 172 to 208 (37 residues), 31.5 bits, see alignment (E = 6.3e-11) PF03486: HI0933_like" amino acids 172 to 207 (36 residues), 26.3 bits, see alignment (E = 1.8e-09) PF01134: GIDA" amino acids 172 to 201 (30 residues), 24 bits, see alignment (E = 1.1e-08) PF12831: FAD_oxidored" amino acids 172 to 209 (38 residues), 39.4 bits, see alignment (E = 2.7e-13) PF13450: NAD_binding_8" amino acids 175 to 213 (39 residues), 27 bits, see alignment (E = 2.5e-09) PF17806: SO_alpha_A3" amino acids 522 to 604 (83 residues), 102.3 bits, see alignment E=8.3e-33 PF01571: GCV_T" amino acids 619 to 883 (265 residues), 247 bits, see alignment E=1.1e-76 PF08669: GCV_T_C" amino acids 910 to 996 (87 residues), 53.3 bits, see alignment E=1.2e-17

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 100% identity to ppf:Pput_0347)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R09 at UniProt or InterPro

Protein Sequence (1004 amino acids)

>PP_0325 sarcosine oxidase subunit alpha (Pseudomonas putida KT2440)
MSQTYRLASGGRIDRSKVLNFTFNGKTYQGYAGDSLAAALLANGVDIVGRSFKYSRPRGI
IAAGTEEPNAILQIGSSEATQIPNVRATQQALYAGLVATSTNGWPNVNNDVMGILGKVGG
SMMPPGFYYKTFMYPKSFWMTYEKYIRKAAGLGRAPLQNDPDSYDYMNRHCDVLIVGAGP
AGLAAALAAARSGARVILADEQEEFGGSLLDTRETLDGKPAADWVNAVVKELEGLPEVTL
LPRATVNGYHDHNFLTIHERLTDHLGDRAPIGQVRHRVHRVRAKRVVLAAGAHERPLVYG
NNDVPGNMLAGAVSTYVRRYGVAPGRKLVLSTNNDHAYRAALDWHDAGLQVVAIADARHN
PRGSLVEEARAKGIRILTSSAVIEAKGSKHVTGARVAAIDVQAHKVTSPGEVLECDLIAS
SGGYSPIVHLASHLGGRPVWRDDILGFVPGDAPQKRECVGGINGVYALGDVIADGFEGGV
RAATEAGFKATVGTLPKTVARKEEATVALFLVPHDKGTKGPKQFVDQQNDVTAAGIELAT
REGFESVEHVKRYTALGFGTDQGKLGNINGLAIAARSIGITIPEMGTTMFRPNYTPVTFG
AVAGRHCGHLFEPVRFTALHAWHVKNGAEFEDVGQWKRPWYFPKAGEDIHAAVTRECKAV
RDSVGLLDASTLGKIDIQGPDAREFLNRIYTNAWTKLDVGKARYGLMCKEDGMVFDDGVT
ACVGDNHFIMTTTTGGAARVLQWMELYHQTEWPELKVYFTSVTDHWATMTLSGPNSRKLL
SELTDIDMDKEAFPFMTWKEGNVGGVPARVFRISFTGELSYEVNVQANYAMGVLEQIIEA
GKKYNLTPYGTETMHVLRAEKGFIIVGQDTDGSMNPDDLNMSWCVGRNKPFSWIGLRGMN
REDCVRENRKQLVGLKPVDPTKWLPEGAQLVFDPKQPIPMDMVGHVTSSYASNSLGYSFA
MGVVKGGLKRMGERVYSPQADGSVIEAEIVSSVFFDPKGERQNV