Protein Info for PP_0320 in Pseudomonas putida KT2440

Annotation: Methyl-accepting chemotaxis transducer

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 290 to 313 (24 residues), see Phobius details PF02743: dCache_1" amino acids 50 to 276 (227 residues), 63 bits, see alignment E=4.8e-21 PF00672: HAMP" amino acids 311 to 362 (52 residues), 54.7 bits, see alignment 1.6e-18 PF00015: MCPsignal" amino acids 460 to 610 (151 residues), 139.9 bits, see alignment E=1.2e-44

Best Hits

Swiss-Prot: 100% identical to MCPH_PSEPK: Methyl-accepting chemotaxis protein McpH (mcpH) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03406, methyl-accepting chemotaxis protein (inferred from 100% identity to ppf:Pput_0342)

Predicted SEED Role

"Methyl-accepting chemotaxis protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R14 at UniProt or InterPro

Protein Sequence (645 amino acids)

>PP_0320 Methyl-accepting chemotaxis transducer (Pseudomonas putida KT2440)
MRIWRKSIQLQLITSMGAALLASILVVVIIFTVALNRLTDRYLVDTALPASIEAIRNDIE
RMLGQPLVAAADIAGNTLLRDWLAAGEDPAQAPQFIEYLTAAKQRNHAFTTLFASTETGH
YYNENGLDRTLSRSNPKDKWFYGYIDSGAERFINIDIDGATGELALFIDYRVEKEGKLVG
VAGMGLRMTELSKLIHDFSFGEHGKVFLVRNDGLIQVHPDAAFSGKRQLAEQLGADAAKG
VMTGGESLRSSRFSRDGERYLALGLPLRDLNWTLVAEVPESEIYAQMHQAVWLTSLIGGA
VALVSLLLVVLLARGLVRPIRRVTAALVQIGSGAGDLSHRLDDSRQDELGDLARGFNRFL
DSQRSLIGEVLSTSERLRRAVEQVTQVVDNTAERSGRQQEMTEMVATAVHEMGLTVQDIA
RNAGDAAQASQSARDEALQAREVVQRSIRGIEGMSGDIGKAADAVSQLADEVASVDEVLA
VIRSISEQTNLLALNAAIEAARAGEMGRGFAVVADEVRTLARRTQLSTDEVQQMIQRLKL
GAGSAVSSMQAGQQATGSGVESSQRTGASLSAITDQVEHISDMNHQVATATEEQSAVTEE
INRTVQGISDLARETAAEVQGCREECQALRGLADDLARQMGGFRL