Protein Info for PP_0290 in Pseudomonas putida KT2440

Annotation: Imidazole glycerol phosphate synthase subunit HisH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01855: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
  • amino acids 4 to 207 (204 residues), 217.8 bits, E=6.2e-69
• PF00117: GATase
  • amino acids 6 to 206 (201 residues), 72.6 bits, E=1.8e-24

Best Hits

Swiss-Prot: 100% identical to HIS5_PSEPK: Imidazole glycerol phosphate synthase subunit HisH (hisH) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02501, glutamine amidotransferase [EC: 2.4.2.-] (inferred from 98% identity to ppg:PputGB1_0315)

Predicted SEED Role

"Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)" in subsystem Histidine Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-

Use Curated BLAST to search for 2.4.2.-

Sequence Analysis Tools

See Q88R44 at UniProt or InterPro

Protein Sequence (212 amino acids)

>PP_0290 Imidazole glycerol phosphate synthase subunit HisH (Pseudomonas putida KT2440)
MQTVAVIDYGMGNLHSVAKALEHVGAGKVLVTSDASVIREADRVVFPGVGAIRDCMAEIR
RLGFDSLVREVSQDRPFLGICVGMQALLEHSEENAGVDCIGLFPGQVRFFGKDLQEEGEH
LKVPHMGWNEVSQTIDHPLWHDIPDRARFYFVHSYYINAGKPGQVVGRGHYGVDFAAALA
DGSRFAVQFHPEKSHTHGLQLLQNFIAWDGRW