Protein Info for PP_0286 in Pseudomonas putida KT2440

Annotation: adenine glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 TIGR01084: A/G-specific adenine glycosylase" amino acids 5 to 275 (271 residues), 372.5 bits, see alignment E=7.5e-116 PF00730: HhH-GPD" amino acids 35 to 166 (132 residues), 75.8 bits, see alignment E=5e-25 PF00633: HHH" amino acids 100 to 128 (29 residues), 35.9 bits, see alignment (E = 6.4e-13) PF14815: NUDIX_4" amino acids 237 to 343 (107 residues), 74.5 bits, see alignment E=9.3e-25

Best Hits

Swiss-Prot: 54% identical to MUTY_SALTY: Adenine DNA glycosylase (mutY) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03575, A/G-specific adenine glycosylase [EC: 3.2.2.-] (inferred from 100% identity to ppu:PP_0286)

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R48 at UniProt or InterPro

Protein Sequence (355 amino acids)

>PP_0286 adenine glycosylase (Pseudomonas putida KT2440)
MSPEQFSSAVLDWYDEHGRHDLPWQQGITPYRVWVSEIMLQQTQVSTVLNYFDRFMQALP
TVQALAEAPEDEVLHLWTGLGYYTRARNLQKAAKVVVEQHGGEFPRSVEQLTELPGIGRS
TAGAIASISMGIRAPILDGNVKRVLARYTAQAGYPGEPKVANQLWATAERFTPQQRANHY
TQAMMDMGATLCTRSKPSCLICPLQRGCEAHLHGEETRYPEPKPRKALPQRRTLMPLLAN
HEGAILLYRRPSSGLWGGLWSLPELDDIAQLDDLAYQHGLRLAGRHAMDGLTHTFSHFQL
AIEPWLVHVDPVGEHVAEADWLWYNLATPPRLGLAAPVKKLLERAADELIAGDAR