Protein Info for PP_0233 in Pseudomonas putida KT2440

Annotation: taurine ABC transporter periplasmic binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF04069: OpuAC" amino acids 26 to 232 (207 residues), 74.3 bits, see alignment E=2.6e-24 TIGR01729: taurine ABC transporter, periplasmic binding protein" amino acids 28 to 320 (293 residues), 436.6 bits, see alignment E=2.5e-135 PF13379: NMT1_2" amino acids 29 to 234 (206 residues), 55.2 bits, see alignment E=2e-18 PF09084: NMT1" amino acids 61 to 242 (182 residues), 75.9 bits, see alignment E=8.7e-25

Best Hits

Swiss-Prot: 56% identical to TAUA_ECOLI: Taurine-binding periplasmic protein (tauA) from Escherichia coli (strain K12)

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 100% identity to ppu:PP_0233)

MetaCyc: 56% identical to taurine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-64-RXN [EC: 7.6.2.7]

Predicted SEED Role

"Taurine-binding periplasmic protein TauA" in subsystem Taurine Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RA0 at UniProt or InterPro

Protein Sequence (323 amino acids)

>PP_0233 taurine ABC transporter periplasmic binding subunit (Pseudomonas putida KT2440)
MIPHAPLRLFAALTLASTSWLAQAADLTVAYQTTVDPAKVAQVDGDYEKASKASIDWRKF
DNGADVITAVASGDVQIGYLGSSPLAAAATRKLPVETFLIATQIGAGEALVARDSIKSPQ
DLIGKKVAVPFVSTGHYSLLAALKSWNIDPSKVQILNLAPPAIIAAWKRGDIDATYVWDP
ALGVAKENGKVLITSGELAQKGAPTFDAWIVRKDFAEKHPDVVKAFAKVTLDAYADYRKD
PKAWLADKGNVDKLVKLSGAKASDIPVLLEGNVYPLAADQANALGAPTTQALTDTANFLK
QQGKVEAVLPDYSPYVSAKFLPN