Protein Info for PP_0213 in Pseudomonas putida KT2440
Annotation: succinate-semialdehyde dehydrogenase (NADP+)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to DAVD_PSEPK: Glutarate-semialdehyde dehydrogenase (davD) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K14269, glutarate semialdehyde dehydrogenase [EC: 1.2.1.20] (inferred from 100% identity to ppg:PputGB1_0237)MetaCyc: 91% identical to NAD(P)-dependent succinate-semialdehyde dehydrogenase (Pseudomonas fluorescens)
Succinate-semialdehyde dehydrogenase (NAD(P)(+)). [EC: 1.2.1.16]
Predicted SEED Role
"Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)" (EC 1.2.1.16, EC 1.2.1.20)
MetaCyc Pathways
- L-lysine degradation IV (5/5 steps found)
- L-lysine degradation X (5/6 steps found)
- 4-aminobutanoate degradation III (2/2 steps found)
- L-lysine degradation I (5/7 steps found)
- L-lysine degradation III (2/6 steps found)
- superpathway of L-lysine degradation (23/43 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.16
Use Curated BLAST to search for 1.2.1.16 or 1.2.1.20
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88RC0 at UniProt or InterPro
Protein Sequence (480 amino acids)
>PP_0213 succinate-semialdehyde dehydrogenase (NADP+) (Pseudomonas putida KT2440) MQLKDAQLFRQQAYINGEWLDADNGQTIKVTNPATGEVIGTVPKMGTAETRRAIEAADKA LPAWRALTAKERSAKLRRWFELMIENQDDLARLMTTEQGKPLAEAKGEIAYAASFIEWFA EEAKRIYGDTIPGHQPDKRLIVIKQPIGVTAAITPWNFPAAMITRKAGPALAAGCTMVLK PASQTPYSALALVELAHRAGIPAGVLSVVTGSAGEVGGELTGNSLVRKLSFTGSTEIGRQ LMEECAKDIKKVSLELGGNAPFIVFDDADLDKAVEGAIISKYRNNGQTCVCANRIYVQDG VYDAFAEKLAAAVAKLKIGNGLEEGTTTGPLIDGKAVAKVQEHIEDAVSKGAKVLSGGKL IEGNFFEPTILVDVPKTAAVAKEETFGPLAPLFRFKDEAEVIAMSNDTEFGLASYFYARD MSRVFRVAEALEYGMVGINTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIKYLCISV