Protein Info for PP_0197 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 195 transmembrane" amino acids 15 to 36 (22 residues), see Phobius details amino acids 60 to 88 (29 residues), see Phobius details amino acids 90 to 117 (28 residues), see Phobius details PF00924: MS_channel_2nd" amino acids 105 to 176 (72 residues), 41.3 bits, see alignment E=6.6e-15

Best Hits

KEGG orthology group: None (inferred from 99% identity to ppf:Pput_0218)

Predicted SEED Role

"Small-conductance mechanosensitive channel"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RD5 at UniProt or InterPro

Protein Sequence (195 amino acids)

>PP_0197 conserved protein of unknown function (Pseudomonas putida KT2440)
MEELRSLLPGQWMDTFWLGLQILLILAAAFILQRIVARGLSRLGQRYPLPPELLVPVRGG
LRWLIMGSALLFVLERMGVSATVLWTALSGFVAVAAVAFFAMWSVLSNLLCAVLIFTVGP
FRIGDVVELVDTLDKPGVKGRVIAINLLFTTLLEVPEAGGALVQVPNSQFFQKSVRRWRG
AEVQALHKETAAEAD