Protein Info for PP_0185 in Pseudomonas putida KT2440

Annotation: Positive alginate biosynthesis regulatory protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 PF00072: Response_reg" amino acids 3 to 113 (111 residues), 81.5 bits, see alignment E=5.1e-27 PF04397: LytTR" amino acids 148 to 244 (97 residues), 73.9 bits, see alignment E=1.1e-24

Best Hits

Swiss-Prot: 79% identical to ALGR_PSEAE: Positive alginate biosynthesis regulatory protein (algR) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K08083, two-component system, LytT family, response regulator AlgR (inferred from 100% identity to ppu:PP_0185)

Predicted SEED Role

"Autolysis response regulater LytR" in subsystem Murein hydrolase regulation and cell death

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RE6 at UniProt or InterPro

Protein Sequence (247 amino acids)

>PP_0185 Positive alginate biosynthesis regulatory protein (Pseudomonas putida KT2440)
MNVLIVDDEPQGRERLTRLLGELEGYTVLEPSATNGDEALALIESLKPDVVLLDIGMPGL
DGLQVAARLCEREAPPAVVFCSGDDEYGAEAFKDSTLSHVTKPFQAHALRDALRKAEKPN
RAQLAALTRPANEGGGPRSHISARTRKGIELIPLPQVIYFIADHKYVTLRHEAGEVLLDE
PLKALEDEFGERFVRIHRNALVARERIERLQRTPLGHFQLYLKGLDGDALTVSRRHVAGV
RKMMQTL