Protein Info for PP_0179 in Pseudomonas putida KT2440

Annotation: putative efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 9 to 465 (457 residues), 276.8 bits, see alignment E=1.7e-86 PF02321: OEP" amino acids 65 to 254 (190 residues), 69.1 bits, see alignment E=2.3e-23 amino acids 285 to 456 (172 residues), 80.3 bits, see alignment E=8.4e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0179)

Predicted SEED Role

"Outer membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RF1 at UniProt or InterPro

Protein Sequence (472 amino acids)

>PP_0179 putative efflux transporter (Pseudomonas putida KT2440)
MKQLILAGLCLSLGACMMVGPDYEVPGDAAVQRNDLNGPLRQDADSVVSAPVPEDWWQLY
QDQRLNELVRQALSANTELRVAAANIAKARAQVEVAESQGGFNGGVKLGAQRLQESGEAF
LQPEKVPVANIGEAIISASYQFDLWGTFKRGTEAAKANADAVQAAADTARITLVADVVKA
YTQVCSANEEYHIARESLDLQEQSVKLNQRLRDAGRGDETQVTRSQTQFKSLRAELPRFK
AERETGMYTLAALLAKPVDQLPAGTADCAELPHLNQLVPVGDGAALLKRRPDVRQAERQL
AAATAYIGVATGALYPDISIGAQVGTIGILENLGEPSTNRWGFGPQISWSIPTNGTRARI
RMAEASTQAALAHFDGVVLNAIRETQTRLAQYSALLDRRDALAEAEKSAKEAADQTHRYY
QAGRESFLADLQATRTYTDMRAQLAAANSQVAQGQIAVFLALGGGWKGTAKP