Protein Info for PP_0176 in Pseudomonas putida KT2440

Annotation: putative efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 transmembrane" amino acids 14 to 34 (21 residues), see Phobius details amino acids 61 to 80 (20 residues), see Phobius details amino acids 86 to 105 (20 residues), see Phobius details amino acids 113 to 130 (18 residues), see Phobius details amino acids 142 to 162 (21 residues), see Phobius details amino acids 367 to 388 (22 residues), see Phobius details amino acids 394 to 411 (18 residues), see Phobius details amino acids 420 to 440 (21 residues), see Phobius details amino acids 446 to 464 (19 residues), see Phobius details amino acids 470 to 490 (21 residues), see Phobius details amino acids 502 to 522 (21 residues), see Phobius details PF04632: FUSC" amino acids 13 to 673 (661 residues), 545.6 bits, see alignment E=2.1e-167 PF13515: FUSC_2" amino acids 26 to 157 (132 residues), 59.3 bits, see alignment E=4.3e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0176)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RF4 at UniProt or InterPro

Protein Sequence (695 amino acids)

>PP_0176 putative efflux transporter (Pseudomonas putida KT2440)
MNGFFSSVPPARDWFYGVRTFGASMIALYIALLMQLPRPYWAMATVYIVSSPFVGPTSSK
ALYRAVGTLLGAGGAIFLVPPLVQSPLLLSIAVALWTGTLLFLSLNLRTANNYVLMLAGY
TLPMIALAVVDNPLAVFDVASSRAQEICLGIVCAAVVGAIFWPRRLAPVVVGATGNWFNE
AIRYSDTYLARDASADKVGGMRGAMVTTFNSLELMIGQLAHEGAGPHTLKNARELRGRMI
HLLPVIDALDDALVALEGRAPAQFAQLQPVLEAAREWLKGTADSASVAHWATLHEQIGRL
QPATAALDQRAELLLSNALYRLTEWADLWQDCCTLQHALRTDDAKPWRAVYRHWRLGRLT
AFFDRGLMLYSVASTVLAIVVACGLWIGLGWNDGASAVILAAVACSFFAAMDDPAPQIYR
FFFWTLMSVIFSSLYLFLVLPNLHDFPMLVLAFAVPFICIGTLTVQPRFYLGTLLTIVNT
STFISIQGAYDADFFTFLNSNLAGPAGLLFAFIWTLVMRPFGVELAAKRMTRFAWRDIVE
MTEPATLAEHRQVGVQMLDRLMQHLPRLSQTGQDSGVALRDLRVGLNLLDLLAYMPRAGQ
QARERLNTVVEEVGAHYAACLRAGERLHAPAALLRNMERARLALNLDELYERGDARTHLL
HALSGLRLALLPGVEVMLEPAEQPQLPPGLDGAPL