Protein Info for PP_0155 in Pseudomonas putida KT2440

Annotation: pyridine nucleotide transhydrogenase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 35 to 53 (19 residues), see Phobius details amino acids 59 to 82 (24 residues), see Phobius details amino acids 93 to 114 (22 residues), see Phobius details amino acids 136 to 159 (24 residues), see Phobius details amino acids 179 to 197 (19 residues), see Phobius details amino acids 203 to 223 (21 residues), see Phobius details amino acids 229 to 247 (19 residues), see Phobius details amino acids 253 to 272 (20 residues), see Phobius details signal peptide" amino acids 24 to 24 (1 residues), see Phobius details PF02233: PNTB" amino acids 7 to 475 (469 residues), 632 bits, see alignment E=3.3e-194

Best Hits

Swiss-Prot: 61% identical to PNTB_RHORT: NAD(P) transhydrogenase subunit beta (pntB) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K00325, NAD(P) transhydrogenase subunit beta [EC: 1.6.1.2] (inferred from 100% identity to ppg:PputGB1_0171)

Predicted SEED Role

"NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RH4 at UniProt or InterPro

Protein Sequence (478 amino acids)

>PP_0155 pyridine nucleotide transhydrogenase, beta subunit (Pseudomonas putida KT2440)
MSMNLVTLLYLVASVCFIQALKGLSHPTTSRRGNLFGMIGMGLAILTTVGLIYKLGAELA
TAGIGYVIVGLLVGGTAGSIMAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVLEPQSMG
IVAAISDPIPTGNRLELFLGAAIGAITFSGSVIAFGKLSGKYKFRLFQGAPVQFAGQHKL
NLILGLTTIALGLLFTFTGHYSAFTLMLVLAFIMGVLIIIPIGGADMPVVVSMLNSYSGW
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGDTDTGAAQGS
QEQRPVKSGSADDATFLLSNADSVIIVPGYGLAVARAQHALKELTEKLTHNGVTVKYAIH
PVAGRMPGHMNVLLAEAEVPYDQVFEMEDINAEFGQADVVLVLGANDVVNPAAKNDPKSP
IAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVE