Protein Info for PP_0154 in Pseudomonas putida KT2440

Updated annotation (from data): Succinyl-CoA:acetate CoA-transferase (EC 2.8.3.18)
Rationale: Specifically important in carbon source Sodium acetate; carbon source Potassium acetate. 48% identical to B3EY95 from Acetobacter, which uses succinyl-CoA to form acetyl-CoA and aids growth on acetate, see PMID:18502856
Original annotation: propionyl-CoA:succinate CoA transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02550: AcetylCoA_hydro
  • amino acids 11 to 215 (205 residues), 103.3 bits, E=2.8e-33
• TIGR03458: succinate CoA transferase
  • amino acids 12 to 494 (483 residues), 775.4 bits, E=1e-237
• PF13336: AcetylCoA_hyd_C
  • amino acids 322 to 463 (142 residues), 141.4 bits, E=3.6e-45

Best Hits

KEGG orthology group: K01067, acetyl-CoA hydrolase [EC: 3.1.2.1] (inferred from 100% identity to ppg:PputGB1_0170)

Predicted SEED Role

"Acetyl-CoA hydrolase (EC 3.1.2.1)" (EC 3.1.2.1)

MetaCyc Pathways

• TCA cycle VII (acetate-producers) (8/9 steps found)
• pyruvate fermentation to acetate V (3/3 steps found)
• acetate formation from acetyl-CoA (succinate) (1/1 steps found)
• pyruvate fermentation to acetate VI (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• succinate fermentation to butanoate (1/7 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.3.18 or 3.1.2.1

Sequence Analysis Tools

See Q88RH5 at UniProt or InterPro

Protein Sequence (497 amino acids)

>PP_0154 Succinyl-CoA:acetate CoA-transferase (EC 2.8.3.18) (Pseudomonas putida KT2440)
MYRDRIRLSSLHSKVMSAADAAGLIEDGMTVGMSGFTRAGEAKAVPHALAERAKQSPLKI
SLMTGASLGNDLDKQLTEAGVLARRMPFQVDSTLRKAINDGQVMFIDQHLSETVEQLRNQ
QLKLPDIAVIEAVAITEQGHIVPTTSVGNSASFAIFAKQVIVEINLSHNTNLEGLHDIYI
PTYRPTRTPIPLVKVDDRIGSTAIPIDPAKIVGIVISDQPDSPSTVLPPDDETQGIADHL
INFLKKEVDAGRMTNKLGPLQAGIGSIANAVMCGLIESPFEDLTMYSEVLQDSTFDLIDA
GKLSFASGSSITLSTRRNADVFGNLERYKDKLVLRPQEISNHPEVVRRLGIIGINTALEF
DIYGNVNSTHVCGTRMMNGIGGSGDFARNAHLAVFVTKSIAKGGAISSVVPMVSHVDHTE
HDVDILVTEQGLADLRGLAPRERARAIIDNCVHPDYRAALNDYFDRACQRGGHTPHILRE
ALSWHENLEETGRMLAS