Protein Info for PP_0129 in Pseudomonas putida KT2440

Annotation: GGDEF domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 PF20975: DGCcoil" amino acids 4 to 467 (464 residues), 247.7 bits, see alignment E=3e-77 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 483 to 643 (161 residues), 158.7 bits, see alignment E=5.6e-51 PF00990: GGDEF" amino acids 485 to 641 (157 residues), 154.1 bits, see alignment E=4.2e-49

Best Hits

KEGG orthology group: K13590, diguanylate cyclase (inferred from 100% identity to ppu:PP_0129)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RK0 at UniProt or InterPro

Protein Sequence (645 amino acids)

>PP_0129 GGDEF domain protein (Pseudomonas putida KT2440)
MTEDAERWKEKYLKSIEQQEKLERRWDARLDLLRRGLVRSTLAAEGSDKVVDECMKEMRD
VIRSDNMDAGLAGLIPRLEKAVLDSEQRRETRMNQVSDALTALVGQLQGLPLPSDISRPL
KKLAKKLEGGVAQSRELPPLLGELSGLQGRALSALGKPSEEARPGFLQRLFGSREEDAQA
GAELAPVALPAAEVVPQAAGSAEPSRHDDVDALQPLPAPAVPTAEVAEAPELELEAFGPA
LIETAEMPPAPLPRDKTPLPEAEVSASESLAEAEPLQALEETFGEDGPYALPYAVEPPYS
QVAAHIEQTLIGLLDDLSLPERHKAQAQEMRERVARGLNWYELIPVLDDLAVLMLAITDS
GQHEFETYLQQLNERLEGFQSHLHEASAGHADNSTAARELDSQLREHVDGLQSSVQGAAD
VDSLKHILENRLEGLLVTMDEHKHERDRREQELAGRLQGLSERVASMEHEALGYREHLEE
QRQKALLDPLTGLPNRAAWSERVEREMLDWQENGGHLAMAILDLDHFKRINDNYGHLAGD
KVLKIVADQLRKRLRGRDFIARFGGEEFVMLLPQTTPAVAAQVAEVLRATVEACPFHFKG
ERVVITTSIGLGAFRSGERSDQVLKRADAALYRAKELGRNRVEHG