Protein Info for PP_0113 in Pseudomonas putida KT2440

Annotation: methionine ABC transporter, permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 transmembrane" amino acids 23 to 46 (24 residues), see Phobius details amino acids 58 to 84 (27 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 152 to 175 (24 residues), see Phobius details amino acids 194 to 213 (20 residues), see Phobius details PF00528: BPD_transp_1" amino acids 45 to 211 (167 residues), 61.2 bits, see alignment E=5.6e-21

Best Hits

Swiss-Prot: 49% identical to METP_BACSU: Methionine import system permease protein MetP (metP) from Bacillus subtilis (strain 168)

KEGG orthology group: K02072, D-methionine transport system permease protein (inferred from 100% identity to ppg:PputGB1_0128)

MetaCyc: 51% identical to L-methionine/D-methionine ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
RXN0-4522 [EC: 7.4.2.11]; 7.4.2.11 [EC: 7.4.2.11]; TRANS-RXN-383 [EC: 7.4.2.11]; TRANS-RXN0-510 [EC: 7.4.2.11]; TRANS-RXN0-511 [EC: 7.4.2.11]

Predicted SEED Role

"Methionine ABC transporter permease protein" in subsystem Methionine Biosynthesis or Methionine Degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RL6 at UniProt or InterPro

Protein Sequence (223 amino acids)

>PP_0113 methionine ABC transporter, permease protein (Pseudomonas putida KT2440)
MDALNFFANVDWAEIWLATVDTMIMLFGSLFFTVLLGLPLGVLLFLCGPKQMFEQKGVYA
LLSLVVNILRSLPFIILLIVMIPFTVLITGTSLGVAGAIPPLVVGATPFFARLVETALRE
VDRGIIEATQSMGATTRQIITSALLPEARPGIFAAITVTAITLVSYTAMAGVVGAGGLGD
LAIRFGYQRFQTDVMVVTVVLLLVLVQVLQSVGDKLVVHFSRK