Protein Info for PP_0088 in Pseudomonas putida KT2440

Annotation: putative alkanal monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 TIGR03558: luciferase family oxidoreductase, group 1" amino acids 9 to 330 (322 residues), 430.9 bits, see alignment E=1.6e-133 PF00296: Bac_luciferase" amino acids 14 to 306 (293 residues), 149.9 bits, see alignment E=5.8e-48

Best Hits

Swiss-Prot: 55% identical to YVBT_BACSU: Uncharacterized protein YvbT (yvbT) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0088)

Predicted SEED Role

"Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RP1 at UniProt or InterPro

Protein Sequence (334 amino acids)

>PP_0088 putative alkanal monooxygenase (Pseudomonas putida KT2440)
MTQLRDLKISTLDLVPVRADAGPAQSLRNSLDLAQHVERFGYNRFWVAEHHNMDGIASSA
TAVLIGYLAGGTSRIRVGSGGVMLPNHAPLVIAEQFGTLASLYPGRIDLGLGRAPGSDQM
TAYALRRDRAGGPDDFPDDVEELSRYLGPRTDDQKVIAVPGYDTDVPMWLLGSSLFSAQL
AGMRGMPYAFASHFAPRYMHEAIRVYRNHFKPSTTLDKPYVMLGIPMVVAQTDEKAEYLA
TSVYQRILALIRGQSLMQKPPVESMDGLWLPHERETVGSFLGLAMIGSPRKVKAKVEVLL
EQTGADELIFTCDLYEHADRIRSYELMAQALKTE