Protein Info for PP_0084 in Pseudomonas putida KT2440

Annotation: HTH-type transcriptional regulator TrpI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 PF00126: HTH_1" amino acids 8 to 66 (59 residues), 77.5 bits, see alignment E=6e-26 PF03466: LysR_substrate" amino acids 96 to 293 (198 residues), 71.2 bits, see alignment E=8.5e-24

Best Hits

Swiss-Prot: 80% identical to TRPI_PSESY: HTH-type transcriptional regulator TrpI (trpI) from Pseudomonas syringae pv. syringae

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0084)

Predicted SEED Role

"Glycine cleavage system transcriptional activator" in subsystem Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RP5 at UniProt or InterPro

Protein Sequence (298 amino acids)

>PP_0084 HTH-type transcriptional regulator TrpI (Pseudomonas putida KT2440)
MAHDLPPLNALRAFEATARLNSVSQAAEALHVTHGAVSRQIKVLEEHLGVALFVKDGRGI
KLTDAGMRLRDASGEAFDRLRSVCSELSRDVSEAPFVLGCSGSLLARWFIPRLGRLQADL
PELRLHLSAGEGDLDPRRPGLDALLVYAEPPWPADMQVHVLAEECIGPVLSPHFAGFQRL
QGAPAKALLGEALLHTTSRPQAWPTWVVQQGLEPAAIHYGQAFEHLYYLLEAAVAGLGVA
IAPQPLVADDLRAGRLSAPWGFSPTQAALALWVPRRAADGRAEQLAQWLRAELARQSA