Protein Info for PP_0044 in Pseudomonas putida KT2440

Annotation: cobalt-zinc-cadmium resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 91 to 404 (314 residues), 169.4 bits, see alignment E=4.8e-54 PF16576: HlyD_D23" amino acids 102 to 331 (230 residues), 120.7 bits, see alignment E=5.5e-39 PF13437: HlyD_3" amino acids 229 to 328 (100 residues), 59.2 bits, see alignment E=6e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppg:PputGB1_0058)

MetaCyc: 100% identical to cobalt-zinc-cadmium resistance protein (Pseudomonas putida KT2440)
RXN1G01-61; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RT5 at UniProt or InterPro

Protein Sequence (416 amino acids)

>PP_0044 cobalt-zinc-cadmium resistance protein (Pseudomonas putida KT2440)
MDNKRKIALAVAAVAALGFGSLAWNNSSGQQAASTAEHGANDGHGDEKKAASAAKEEGDE
GHGEEGHSEEGGEEEGKLTLSAEQIKAAGVALEAAAPRDLGTVVSFPGEIRFDEDRTAHV
VPRVPGVVEAVQANLGETVKKGQVLAVIASQQISDLRSEQQAAQRRVELARVTFEREKQL
WQDKISAEQDYLQARQALQEAEISLANAKQKVGAIGASVNSVGGNRYELRAPFDAVVVEK
HLTVGEVVSEATNAFILSDLNQVWATFAVPPTDLGKVTTGRAVKVSSPDMNVEVEGKVGY
VGSLLGEQNRAATVRVTLTNPNGAWRPGLFVNIAVTSQTDRVAVAVPEHAVQTVEDKPSV
FVRTPDGFDTRPVKLGRRDNGYVEIIDGIEAGAQVATSGSFTLKSELGKASAEHGH