Protein Info for PP_0041 in Pseudomonas putida KT2440

Annotation: cadmium translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 665 transmembrane" amino acids 30 to 48 (19 residues), see Phobius details amino acids 55 to 72 (18 residues), see Phobius details amino acids 92 to 116 (25 residues), see Phobius details amino acids 273 to 292 (20 residues), see Phobius details amino acids 304 to 327 (24 residues), see Phobius details amino acids 617 to 636 (20 residues), see Phobius details amino acids 641 to 660 (20 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 87 to 658 (572 residues), 564.8 bits, see alignment E=3.7e-173 PF00122: E1-E2_ATPase" amino acids 138 to 332 (195 residues), 175 bits, see alignment E=1.1e-55 TIGR01494: HAD ATPase, P-type, family IC" amino acids 246 to 388 (143 residues), 82.2 bits, see alignment E=4.3e-27 amino acids 454 to 637 (184 residues), 125.3 bits, see alignment E=3.4e-40 PF00702: Hydrolase" amino acids 350 to 568 (219 residues), 122.3 bits, see alignment E=3.4e-39

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to ppw:PputW619_0058)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RT8 at UniProt or InterPro

Protein Sequence (665 amino acids)

>PP_0041 cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
MKSLLERPDDQAGHEGHSHEHGGIFGMNTELIFALICGALLGAGALAGKLGLIDRLPLIL
YVSAYVFGGWFTTKEAVTNIRQKRFEIDSLMLLAAVGAASIGAWAEGALLLFLFSLGHSL
ESYAMGRAKKAIEALSKLAPATAIVRRANGTVEMPVELLVPGDVVIVRPNDRLPADGFVV
VGSSSINQAPVTGESVPVDKQPVPDAELARSKPDAVDAASKVFAGTINGETLIEVEVTRR
STESTLARVIKMVSEAEVRKSPTQRFTDRFQRIFVPLVLLLVVGLLFAGIFLDEPFRDSF
YRAMAVLVAASPCALAIATPSAILSGIARAARGGVLIKGGAPLEELGSLNAMAFDKTGTL
TEGRPRITDVIPIGGTQIEDLLNVAIAVESMSDHPLAAAIVRDGEEMIGTRRRFQAKNMS
NMIGRGVRAELDGQFVWIGKVEMFGTNGIPALSKAALEAAERLRQSGRTTMVVRRADKDL
GAIGLLDTPREGAKEALQKLREMGIERMVMISGDHNRVAEAVAKQVGLDEAWGDLMPEDK
VKAIKNLRLSAKVAMVGDGVNDAPAMASSSVGIAMGAAGSDVALETADIALMADDIRQLP
FAVGLSRHTRSIIHQNLFVSLGIVAILVPSTIMGLSIGAAVAIHEGSTLLVVFNALRLLA
YRRST