Protein Info for PP_0030 in Pseudomonas putida KT2440

Annotation: metal-sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 159 to 185 (27 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 3 to 460 (458 residues), 446 bits, see alignment E=9e-138 PF00672: HAMP" amino acids 184 to 236 (53 residues), 25.4 bits, see alignment 2.2e-09 PF00512: HisKA" amino acids 242 to 304 (63 residues), 50.3 bits, see alignment E=3e-17 PF02518: HATPase_c" amino acids 353 to 462 (110 residues), 88.4 bits, see alignment E=6.7e-29

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_0044)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RU9 at UniProt or InterPro

Protein Sequence (472 amino acids)

>PP_0030 metal-sensor histidine kinase (Pseudomonas putida KT2440)
MRPFSLAAKLGLKVGLMSAALLLLFATFGYLMVGKALERNARADLEVKMAGMAHNLSTIP
DISGVNADAHQLVDLVMGHNNLYVSIFDTSQNQTPLLSIGSKQVNLEVHKFQPAAQPKEH
EWRDTQDRPLLSASRIMRLGDGTEVSVYMTMDQSSSEALLDALLTWALMMSPVILALILA
IGWWTVRRGLLPLTKFLKIASKISTESLDHRLPTDGMPAELKMLADGINIMLARLDDGVQ
QLSQFSDDLAHELRAPLSNLMGKAQVALTRERSLSEYREVLESCTEELDRMSRMVSDMLF
LAQVSHPASMVEFESVSLVDEVQRVCDLFSISAEEGEIQLQISGSDDVVRGNRLMVQRAV
SNLVSNAIRYCPRGRTVYVKVQHSASGTTLSVGNPGRGIAKEHHAHLFERFYRVDKSRAR
SEGGTGLGLAIVQSIMSLHQGSASVEVTEENFTWFHLHFPEAQQMQPAMTAA