Protein Info for PFR28_05244 in Pseudomonas sp. RS175

Annotation: Copper-exporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 793 transmembrane" amino acids 156 to 174 (19 residues), see Phobius details amino acids 186 to 207 (22 residues), see Phobius details amino acids 219 to 238 (20 residues), see Phobius details amino acids 244 to 263 (20 residues), see Phobius details amino acids 400 to 419 (20 residues), see Phobius details amino acids 426 to 450 (25 residues), see Phobius details amino acids 740 to 759 (20 residues), see Phobius details amino acids 765 to 785 (21 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 8 to 68 (61 residues), 33.5 bits, see alignment 9.8e-12 amino acids 74 to 135 (62 residues), 43.6 bits, see alignment 6.9e-15 PF00403: HMA" amino acids 11 to 66 (56 residues), 47.1 bits, see alignment 3.8e-16 amino acids 75 to 134 (60 residues), 50.1 bits, see alignment 4.4e-17 TIGR01511: copper-translocating P-type ATPase" amino acids 200 to 783 (584 residues), 588.5 bits, see alignment E=3.8e-180 TIGR01525: heavy metal translocating P-type ATPase" amino acids 218 to 782 (565 residues), 628.6 bits, see alignment E=2.9e-192 TIGR01494: HAD ATPase, P-type, family IC" amino acids 253 to 760 (508 residues), 273.5 bits, see alignment E=6.8e-85 PF00122: E1-E2_ATPase" amino acids 281 to 460 (180 residues), 188.8 bits, see alignment E=1e-59 PF00702: Hydrolase" amino acids 479 to 694 (216 residues), 104.1 bits, see alignment E=2e-33

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 91% identity to pba:PSEBR_a657)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (793 amino acids)

>PFR28_05244 Copper-exporting P-type ATPase (Pseudomonas sp. RS175)
MSESTTFNLPIAGMTCASCAGRVERALSKVAGTRAVSVNLATEQARVEAPAQSLPALMQA
VEHAGYSVPVQTLELKIEGMTCASCVGRVERALGKVPGVNRVSVNLANERAHLQLLGQVD
SQALIDAVKRAGYDASVWQAEPPQDSQIRRLDRERLVLILAIMLSLPLVLPMLLQPFGVH
WMLPAWVQFVLATPVQFIFGARFYVAAFKAVRAGAGNMDLLVALGTSAGYGLSIYEWVLA
GSSHLYFEASAVVIALVLLGKYLESRAKRQTASAIRALEALRPERATQVIDGQERDVAIS
ALRLDDVVLVKPGERFPVDGEVLEGQSHADEALISGESLPVPKQPGDKVTGGAINGEGRL
LVRTRALGTETVLARIIRLVEDAQAAKAPIQKLVDKVSQVFVPVVLVLALATLAGWWLYG
APLETALINAVAVLVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALERAHEVSSVV
FDKTGTLTSGTPRIAHLAALDGDEATLLQLAGALQRGSEHPLAKAVLDACAERGLDVPNV
SDSQALAGRGIAGSLDGRRLALGNRRLLDELGLAPGSLAESAQAWETEGRTLSWLVEQNA
TPRVLGLFAFGDTLKPGALAAVQALDARHITSHLLTGDNRGSARVVSEVLGIANVHAEVL
PGDKSAIVVELKKNAVVAMVGDGINDAPALAAADIGIAMGGGTDVAMHAAGITLMRGDPR
LVPAALDISRKTYAKIRQNLFWAFVYNLIGIPLAAFGLLNPVLAGAAMALSSVSVVSNAL
LLKFWTPGHLEDK