Protein Info for PFR28_05200 in Pseudomonas sp. RS175

Annotation: Cobalt-precorrin-5B C(1)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01888: CbiD
  • amino acids 12 to 271 (260 residues), 292 bits, E=1.7e-91
• TIGR00312: cobalamin biosynthesis protein CbiD
  • amino acids 19 to 317 (299 residues), 198.1 bits, E=9e-63

Best Hits

Swiss-Prot: 89% identical to CBID_PSEPF: Cobalt-precorrin-5B C(1)-methyltransferase (cbiD) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K02188, cobalamin biosynthesis protein CbiD (inferred from 93% identity to pba:PSEBR_a614)

Predicted SEED Role

"Cobalt-precorrin-6 synthase, anaerobic" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)

Protein Sequence (365 amino acids)

>PFR28_05200 Cobalt-precorrin-5B C(1)-methyltransferase (Pseudomonas sp. RS175)
MRDETAEQPAPLRSGLTTGSCATATSLAAARLLLCGISADAVEIVLPKGKHVQMRLEFCR
LTELGAEAGTLKDAGDDPDVTHGALLFSQVRLMPEPGVRFLAGRGVGTVTRPGLVLEVGE
PAINPVPRKMIGDHLGRLAGETGYTGGFEVTVNVEGGEALALKTMNPRLGILGGLSILGT
SGIVRPFSCAAYIASIHQGIDVAKTNGYLHIAACTGNASEDTMRRVYDLPEIALIEMGDF
VGAVLKHVRKVAVEKLSLCGGFGKISKLAAGHMDLHSRHSSIDLPQLAAWAAAVGADEAL
QDDIRHANTSQQALAMASAAGIALGDAVCGHALAFARSIVPAQVQVEVFAIDRQGGIVGH
AGALQ