Protein Info for PFR28_05091 in Pseudomonas sp. RS175

Annotation: Phosphomethylpyrimidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 24 to 101 (78 residues), 110 bits, E=4e-36
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 140 to 592 (453 residues), 721.7 bits, E=1.3e-221
• PF01964: ThiC_Rad_SAM
  • amino acids 142 to 590 (449 residues), 616 bits, E=3.2e-189

Best Hits

Swiss-Prot: 96% identical to THIC_PSEF5: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 99% identity to pba:PSEBR_a512)

MetaCyc: 73% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (629 amino acids)

>PFR28_05091 Phosphomethylpyrimidine synthase (Pseudomonas sp. RS175)
MTTKSKNATNLSDSAQVDQQSVQPFTRSQKIYVQGSRPDIRVPMREISLDVTPTDFGGEI
NAPVTVYDTSGPYTDPNVIIDVRKGLADVRSPWIEARGDTERLAGLSSSFGKERLADPEL
TKLRFAHVNNPRRAKPGANVSQMHYARKGIITAEMEYVAIRENMKLEVARAAGLLDQQHA
GHSFGASIPKVITPEFVRDEIARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALG
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGV
AEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLAH
HKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADANDEAQFGELETLGELTKIAWKH
DVQCMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFE
FRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRI
EAVDVDVAQGMAEQAERFKQEGSQLYKKV