Protein Info for PFR28_05053 in Pseudomonas sp. RS175
Annotation: Bifunctional protein PutA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to PUTA_KLEAE: Bifunctional protein PutA (putA) from Klebsiella aerogenes
KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 66% identity to ajs:Ajs_2420)MetaCyc: 74% identical to trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Salmonella enterica enterica serovar Typhimurium)
RXN-14903 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]
Predicted SEED Role
"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-proline degradation I (3/3 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-Nδ-acetylornithine biosynthesis (5/7 steps found)
- L-arginine degradation I (arginase pathway) (2/3 steps found)
- proline to cytochrome bo oxidase electron transfer (1/2 steps found)
- ethene biosynthesis II (microbes) (1/4 steps found)
- (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1317 amino acids)
>PFR28_05053 Bifunctional protein PutA (Pseudomonas sp. RS175) MATTTLGVKLDDPTRERLKAAATSIDRTPHWLIKQAIFNYLEKLEGGATLTELNGSTRID SDEAGDVQVDHAHQCFLEFAESILPQSVLRASITAAYRRPEPEVVPMLIEQARLPAEMAE ATNKLAASIAEKLRNQKSAGGRAGIVQGLLQEFSLSSQEGVALMCLAEALLRIPDKGTRD ALIRDKISTGNWQPHLGNSPSLFVNAATWGLLLTGKLVATHNEAGLTSSLSRIIGKSGEP MIRKGVDMAMRLMGEQFVTGETIAEALANASKFESKGFRYSYDMLGEAALTEHDAQKYLA SYEQAIHSIGKASHGRGIYEGPGISIKLSALHPRYSRAQYERVMDELYPRLLSLTLLAKQ YDIGLNIDAEEADRLELSLDLLERLCFEPQLTGWNGIGFVIQAYQKRCPYVIDYVIDLAR RSRHRLMIRLVKGAYWDSEIKRAQVEGLEGYPVYTRKVYTDVSYIACARKLLSVPEAIYP QFATHNAHTLSAIYHIAGQNYYPGQYEFQCLHGMGEPLYEQVVGKVSEGKLNRPCRVYAP VGTHETLLAYLVRRLLENGANTSFVNRIADQSISIQELVADPVASIEQMATLEGGFGLPH PRIPLPRDLYGSERANSSGIDMANEHRLASLSCALLATAHNDWKAAPMLGCAASEETPAP VLNPADHRDVVGHVQEATVADVDNAIQCALNAAPIWQATPPAERAAILERAADLMEGEIQ PLMGLLAREAGKTFANAIAEVREAVDFLRYYAVQARNDFTNDAHRPLGPVVCISPWNFPL AIFSGQVAAALAAGNPVLAKPAEQTPLVAAQAVRLMLEAGIPEGVLQLLPGRGETVGARL VGDDRVKGVMFTGSTEVARLLQRNIAGRLDNQGRPIPLIAETGGQNAMIVDSSALTEQVV IDVVSSAFDSAGQRCSALRVLCLQEDSADRVIEMLKGAMAESRLGNPERLSVDIGPVIDA EAKAGIEKHIQAMRDKGRSVYQMAIADSEECKRGTFVMPTLIELESFDELQREIFGPVLH VVRYKRKELDQLIDQINASGYGLTLGVHTRIDETIAKVIDNVHAGNVYVNRNIVGAVVGV QPFGGEGLSGTGPKAGGPLYLYRLLSTRPADAIQQSFVRGDAASAPDLRLRDALSKPLNA LKAWADSQKLAELSALCVQFAAQSQSGITRQLTGPTGERNSYAILPREHVMCLAEVEGDL LTQLAAVLAVGGSAVWPETDTAKALFARLPKDIQARIQRVADWTKDDVVFDAVLHHGDSD QLRSVCQQVAKRPGAIVGVNGLSQGETGIALERLVIERALSVNTAAAGGNASLMTIG