Protein Info for PFR28_05016 in Pseudomonas sp. RS175

Annotation: Glutamate synthase [NADPH] small chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01318: glutamate synthase, small subunit
  • amino acids 9 to 472 (464 residues), 795.4 bits, E=7.1e-244
• PF14691: Fer4_20
  • amino acids 27 to 137 (111 residues), 133.1 bits, E=1.4e-42
• PF07992: Pyr_redox_2
  • amino acids 151 to 459 (309 residues), 93 bits, E=8.1e-30
• PF13450: NAD_binding_8
  • amino acids 154 to 188 (35 residues), 25.4 bits, 5e-09
• PF00070: Pyr_redox
  • amino acids 291 to 364 (74 residues), 27.6 bits, E=1.2e-09

Best Hits

Swiss-Prot: 78% identical to GLTD_HALED: Glutamate synthase [NADPH] small chain (gltD) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 99% identity to pfo:Pfl01_0414)

MetaCyc: 70% identical to glutamate synthase subunit GltD (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]; Glutamate synthase (NADPH). [EC: 1.4.1.4, 1.4.1.13]

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14 or 1.4.1.4

Protein Sequence (472 amino acids)

>PFR28_05016 Glutamate synthase [NADPH] small chain (Pseudomonas sp. RS175)
MAERLSNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEW
KCPVHNFIPNWLKLVAEGNILQAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
TIGSVEKYITDTAFAMGWRPDMSKVKPTGKRVAIIGAGPAGLGCADVLVRGGVTPVVFDK
NPEIGGLLTFGIPEFKLEKTVLSNRREVFTGMGIEFRLNTEVGKDVTMEQLLEEYDAVFM
GMGTYTYMKGGFAGEDLPGVYDALDFLIANVNRNLGFEKSPEDFVDMKGKKVVVLGGGDT
AMDCNRTSIRQGAKSVTCAYRRDEANMPGSRKEVKNAKEEGVKFLYNRQPIAIVGEDKVE
GVKVVETRLGEPDARGRRSPEPIPGSEEIIPADAVVIAFGFRPSPAPWFEQFSIQTDSQG
RVVAPEQGQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV