Protein Info for PFR28_04976 in Pseudomonas sp. RS175

Annotation: Proline iminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 TIGR01249: prolyl aminopeptidase" amino acids 9 to 311 (303 residues), 442.2 bits, see alignment E=4.7e-137 PF00561: Abhydrolase_1" amino acids 36 to 296 (261 residues), 130.6 bits, see alignment E=1.6e-41 PF12146: Hydrolase_4" amino acids 37 to 293 (257 residues), 47.4 bits, see alignment E=3.2e-16 PF12697: Abhydrolase_6" amino acids 37 to 295 (259 residues), 54.1 bits, see alignment E=7.2e-18

Best Hits

Swiss-Prot: 56% identical to PIP_LEPBY: Probable proline iminopeptidase (pip) from Leptolyngbya boryana

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 99% identity to pba:PSEBR_a389)

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>PFR28_04976 Proline iminopeptidase (Pseudomonas sp. RS175)
MQTLYPQIKPHARHDLAVDDTHTLYVDESGSPEGLPVVFIHGGPGAGCDAQSRRYFDPNL
YRIVTFDQRGCGRSTPHASLENNTTWDLVEDLERIRKHLGIDKWVLFGGSWGSTLALAYA
QTHPERVHGLILRGIFLCRPQEIEWFYQAGASRLFPDYWQDYIAPIPPDERDDLLSAFHK
RLTGNDQIAQMHAAKAWSIWEGRTATLRPNPLVVDRFSEPQRALSIARIECHYFTNNAFL
EPNQLIRDMGKIAHLPGIIVHGRYDVICPLDNAWELHQNWPNSELQVIRDAGHAASEPGI
TDALVRAAAQMARRLLDLPPEEA