Protein Info for PFR28_04972 in Pseudomonas sp. RS175

Annotation: Imidazolonepropionase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01224: imidazolonepropionase
  • amino acids 21 to 396 (376 residues), 487.4 bits, E=1.3e-150
• PF01979: Amidohydro_1
  • amino acids 57 to 379 (323 residues), 78.3 bits, E=7e-26
• PF07969: Amidohydro_3
  • amino acids 106 to 375 (270 residues), 53.9 bits, E=2.2e-18

Best Hits

Swiss-Prot: 89% identical to HUTI_PSEPF: Imidazolonepropionase (hutI) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01468, imidazolonepropionase [EC: 3.5.2.7] (inferred from 94% identity to pba:PSEBR_a385)

MetaCyc: 82% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Protein Sequence (400 amino acids)

>PFR28_04972 Imidazolonepropionase (Pseudomonas sp. RS175)
MKTLWQNCHAATMAQGVYSIIEDAAIVTQGEHIQWIGPRAQLPAGDYPAVTDLKGAWVTP
GLIDCHTHTVFGGNRSGEFEQRLQGVSYAEIAAAGGGIASTVRATRAASEDELFASAARR
LESLMHDGVTTVEIKSGYGLDLASERKMLRVIRRLGVELPLSVRSTCLAAHALPPEYVDR
ADDYIEHICAEMLPALAAEGLVDAVDAFCEYLAFSPAQVERVFITAQRLGLPVKLHAEQL
SSLHGSSLAARYQALSADHLEFMTEDDARAMAEAGTVAVLLPGAFYFLRETRLPPMDALR
KHGVKIAVASDLNPGTSPALSLRLMLNMACTCLRMTPEEALAGATIHAAQALGMAASHGS
LEAGKVADFVAWHIDRPADLSYWLGGELEKRVVRHGVEID