Protein Info for PFR28_04874 in Pseudomonas sp. RS175

Annotation: Phosphoenolpyruvate carboxykinase (ATP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 TIGR00224: phosphoenolpyruvate carboxykinase (ATP)" amino acids 6 to 513 (508 residues), 600.6 bits, see alignment E=1.5e-184 PF01293: PEPCK_ATP" amino acids 7 to 467 (461 residues), 637.6 bits, see alignment E=6.1e-196

Best Hits

Swiss-Prot: 96% identical to PCKA_PSEPF: Phosphoenolpyruvate carboxykinase (ATP) (pckA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 96% identity to pfo:Pfl01_0268)

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (513 amino acids)

>PFR28_04874 Phosphoenolpyruvate carboxykinase (ATP) (Pseudomonas sp. RS175)
MTQANNTVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPADRFIVEEPSTQD
AIAWGPINRKFPADKFDALWARVEAFNNAQEHFVSHVHVGASDEHYLAVKMTTQTAWQNL
FGRCLFINPAQYNPAGRQEWQILNVANFECVPERDGTNSDGCVILNFAQKKVLIAGMRYA
GEMKKAMFSVQNFLLPAADVLPMHCAANIGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIKHGAVLENVVLDEAKKADYADGSL
TQNSRAAYPLEHVEKRSAKNLGGEPNAVIFLTCDLTGVLPPVSILSEEQAAYHFLSGYTA
LVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRIRGFGSKVYLVNTGWTGGGY
GVGKRFNIPTTRAVIAAIQSGALVGAATEHLDIINLDVPLAVPGVETGLLNPRNTWADKA
AYDEAAKALAGLFIENFKKFEVSDAIKAAGPQL