Protein Info for PFR28_04824 in Pseudomonas sp. RS175

Annotation: Cadmium-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 transmembrane" amino acids 129 to 148 (20 residues), see Phobius details amino acids 154 to 172 (19 residues), see Phobius details amino acids 184 to 201 (18 residues), see Phobius details amino acids 206 to 224 (19 residues), see Phobius details amino acids 358 to 379 (22 residues), see Phobius details amino acids 388 to 414 (27 residues), see Phobius details amino acids 694 to 713 (20 residues), see Phobius details amino acids 719 to 741 (23 residues), see Phobius details TIGR01511: copper-translocating P-type ATPase" amino acids 171 to 740 (570 residues), 401.7 bits, see alignment E=1.3e-123 TIGR01525: heavy metal translocating P-type ATPase" amino acids 186 to 740 (555 residues), 587.4 bits, see alignment E=7e-180 TIGR01494: HAD ATPase, P-type, family IC" amino acids 208 to 459 (252 residues), 128.3 bits, see alignment E=5.8e-41 amino acids 536 to 722 (187 residues), 139.2 bits, see alignment E=2.8e-44 PF00122: E1-E2_ATPase" amino acids 237 to 418 (182 residues), 180 bits, see alignment E=5.1e-57 PF00702: Hydrolase" amino acids 437 to 650 (214 residues), 121.6 bits, see alignment E=8.2e-39

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 89% identity to pba:PSEBR_a240)

MetaCyc: 75% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (743 amino acids)

>PFR28_04824 Cadmium-transporting ATPase (Pseudomonas sp. RS175)
MSDSLHTHKPVHKSGHGHAHGHSCCASKAAPSVVDLGKAPTDGARLSSFRIEAMDCPTEQ
TLIQNKLGKLEGVQRLEFNLINRILGVTHALPSDAPIIEAIRSLGMQAEPLAAGQDTPTH
TPPSPAKPWWPLALSGLTALGAEVIHFTSAAPNWVVALVALVSILSGGLGTYKKGWIALK
NRNLNINALMSIAVTGAVLIGQWPEAAMVMFLFTVAELIEAKSLDRARNAISGLMQMAPE
QATVQQVDGSWKVQEVKAIALGARVRVRPGERVGLDGEVVAGRSTIDQAPITGESLPVEK
TVGDNVFAGTINQAGELEYTVTAAAEHSTLARIIHAVEQAQGSRAPTQRFVDRFSKIYTP
SVFALAVAVAVIPPLFMGATWFDWIYRALVLLVVACPCALVISTPVSIVSGLAAAARKGI
LIKGGVYLEGGYKLDYLALDKTGTITHGKPVQTDYVALDPTLQASAPALAASLAARSDHP
VSRAIASAVVDKQASPQSVDNFAALPGRGVRGDIDGRTYHLGNHRLVEDLGLCSPELEET
LFALEKQGKSVVLLLDDTGPLALFAVADTVKDSSRDAIRQLHELGIKTLMLTGDNAHTAE
AIAGQVGMDRAQGDLLPEDKLKAIEALYAQGHRVGMVGDGINDAPALARAEIGFAMAAAG
TDTAIETADVALMDDDLRKIPAFIRLSRQTSAILKQNIALALVVKAIFLGVTFTGYATLW
MAVFADMGVSLLVVFNGLRLLRK