Protein Info for PFR28_04728 in Pseudomonas sp. RS175

Annotation: Acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 20 to 132 (113 residues), 139.6 bits, E=1.1e-44
• PF02770: Acyl-CoA_dh_M
  • amino acids 136 to 227 (92 residues), 73.4 bits, E=2.8e-24
• PF00441: Acyl-CoA_dh_1
  • amino acids 240 to 385 (146 residues), 99 bits, E=6.2e-32
• PF08028: Acyl-CoA_dh_2
  • amino acids 254 to 374 (121 residues), 27.7 bits, E=5.6e-10

Best Hits

Swiss-Prot: 68% identical to GCDH_DICDI: Glutaryl-CoA dehydrogenase, mitochondrial (gcdh) from Dictyostelium discoideum

KEGG orthology group: K00252, glutaryl-CoA dehydrogenase [EC: 1.3.99.7] (inferred from 99% identity to pba:PSEBR_a156)

MetaCyc: 95% identical to glutaryl-CoA dehydrogenase (Pseudomonas putida KT2440)
GLUTARYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.6]

Predicted SEED Role

"Glutaryl-CoA dehydrogenase (EC 1.3.99.7)" (EC 1.3.99.7)

MetaCyc Pathways

• glutaryl-CoA degradation (3/5 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• L-tryptophan degradation III (eukaryotic) (3/15 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Fatty acid metabolism
• Lysine degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.6 or 1.3.99.7

Protein Sequence (393 amino acids)

>PFR28_04728 Acyl-CoA dehydrogenase (Pseudomonas sp. RS175)
MAVKASFNWIDPLLLDQQLTEEERMIRDTAEQFAQQKLAPRVLEAFRHEKTDPAIFREMG
DMGLLGATIPEQYGGSGLNYVSYGLIAREVERVDSGYRSMMSVQSSLVMVPINEFGTEAQ
KQKYLPKLASGEWIGCFGLTEPNHGSDPGAMITRARKVEGGYSLTGSKMWITNSPIADVF
VVWGKDDAGDIRGFVLEKGWKGLSAPAIHGKVGLRASITGEIVMDNVFVPEENIFPDVRG
LKGPFTCLNSARYGIAWGALGAAEFCWHTARQYTLDRQQFGRPLAANQLIQKKLADMQTE
ITLALQGCLRLGRMKDEGTAAVEITSIMKRNSCGKSLEIARMARDMLGGNGISDEFGVAR
HLVNLEVVNTYEGTHDVHALILGRAQTGIQAFY