Protein Info for PFR28_04652 in Pseudomonas sp. RS175

Annotation: Choline-sulfatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details TIGR03417: choline-sulfatase" amino acids 9 to 504 (496 residues), 858.9 bits, see alignment E=6.6e-263 PF00884: Sulfatase" amino acids 10 to 350 (341 residues), 168 bits, see alignment E=6.6e-53 PF16347: SGSH_C" amino acids 300 to 436 (137 residues), 43.4 bits, see alignment E=8.8e-15 PF12411: Choline_sulf_C" amino acids 451 to 503 (53 residues), 89.7 bits, see alignment 1.7e-29

Best Hits

KEGG orthology group: None (inferred from 95% identity to pba:PSEBR_a65)

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>PFR28_04652 Choline-sulfatase (Pseudomonas sp. RS175)
MECREHMKRKNILFIMADQMAAPMLPIYGSSPIKLPNLSRLADQGVVFDAAYCNSPLCAP
SRFTLVSGQLPSKIGAYDNAADFPADVPTYAHYLRRLGYRTALSGKMHFCGPDQLHGYEE
RLTSDIYPADYGWAVNWDEPDVRPSWYHNMSSVLQAGPCVRTNQLDFDEEVVFKAQQYLF
DHIREDGDQPFCLTVSMTHPHDPYTIPKTFWDLYEDKDIPLPHTPAQSELDPHSQRLLKV
YDLWDKPLPVDKIRDARRAYFGACSYIDSNIGKLLQTLEDTGLMDDTIIIFSGDHGDMLG
EKGLWYKMHWFEMAARVPLLISAPGQFASGRVGAAVSTADLLPTLVELAGGTLEPGLPLD
GRSLVPHLQGQGGHDEVFGEYMAEGTISPLMMIRRGPWKFIYSEDDPCLLFNVRNDPREL
EDLSQSPDHQQLFADFLAEARAKWDIPGIHRQVLASQRRRRFVAQALTLGKLKSWDHQPL
VDASQQYMRNHIDLDDLERKARYPQPCQNQ