Protein Info for PFR28_04624 in Pseudomonas sp. RS175

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 922 TIGR00593: DNA polymerase I" amino acids 6 to 922 (917 residues), 933.3 bits, see alignment E=9.5e-285 PF02739: 5_3_exonuc_N" amino acids 6 to 167 (162 residues), 180.2 bits, see alignment E=5.4e-57 PF01367: 5_3_exonuc" amino acids 168 to 270 (103 residues), 104.4 bits, see alignment E=7.9e-34 PF01612: DNA_pol_A_exo1" amino acids 315 to 509 (195 residues), 95.7 bits, see alignment E=5.7e-31 PF00476: DNA_pol_A" amino acids 546 to 920 (375 residues), 540.7 bits, see alignment E=3.7e-166

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 95% identity to pba:PSEBR_a40)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (922 amino acids)

>PFR28_04624 DNA polymerase I (Pseudomonas sp. RS175)
MSQAPLVLVDGSSYLYRAFHALPPLTTSKGLPTGAVKGVLNMLKSLRKQYPDSPFAVVFD
AKGGTFRDALYAEYKANRPSMPDDMRVQIEPLHASVKALGFPLLCVDNVEADDVIGTLAR
SSAAADRPVVISTGDKDMAQLVDGHITLVNTMTGSSLDVAGVKEKFGVAPEQIIDYLALM
GDSSDNIPGVPGIGPKTAAGLLVGVNGGLTELYAQLDIVPTLPIRGAKTLPAKLEEHKEM
AFLSYELATIKTDVPLDIGLDDLQMGAPDHEKLAELYTLLEFKSWFEENQRDAKRAGQEI
VEIADEHPAGAEAKYEVILDQARFDAWLAKLEQAPLFAFVTQTNGGDAQQAQLVGLSFSV
AAFEAAYIPLTHSYMGVPEQLDRDTVLKALKPLLENPDKPKVGQHAKFETNILANCAIGG
DQGNGIMVQGIAFDTMLESYVLDSTATRHDMDSLALKYLGQSKTDLQDIAGKGVKQLSFD
QIALELAGPYAAEDADVTFRLHQALQERLVAIPSLNKVLTEIEMPLMPVLARIERQGALV
DANLLGVQSVELGEKLVALEREAFAIAGEEFNLGSPKQLGVILYEKLGLPVLSKTAKGQA
STAEAVLAELAEQDYPLPKVLMQYRSLSKLKSTYTDRLPEQINSRTGRVHTNYQQAVAAT
GRLSSIDPNLQNIPIRTAEGRRIRQAFVAPKGYKLLAADYSQIELRIMAHLAKDEGLLHA
FRNDLDVHRATAAEVFGVDLDAVTHDQRRSAKAINFGLIYGMSAFGLAKQIGVDRKQSQA
YIDRYFARYPGVLAYMERTRTQAAEQGFVETIFGRRLYLPDINAKNPALRKGAERTAINA
PMQGTAADIIKKAMVAVDRWLTASGLDAKVILQVHDELVLEVREDLVDQVREDIRRHMSA
AATLDVPLLVEVGVGNNWDEAH