Protein Info for PFR28_04557 in Pseudomonas sp. RS175

Annotation: Vitamin B12 import ATP-binding protein BtuD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 PF00005: ABC_tran" amino acids 41 to 173 (133 residues), 81.3 bits, see alignment E=1.1e-26 PF14524: Wzt_C" amino acids 261 to 398 (138 residues), 123.2 bits, see alignment E=8.4e-40

Best Hits

KEGG orthology group: K09691, lipopolysaccharide transport system ATP-binding protein (inferred from 93% identity to pba:PSEBR_a5631)

MetaCyc: 62% identical to O:8-antigen ABC transporter ATP-binding subunit (Escherichia coli O8)
TRANS-RXN-445 [EC: 7.5.2.14]

Predicted SEED Role

"Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40)" in subsystem Rhamnose containing glycans or Teichoic and lipoteichoic acids biosynthesis (EC 3.6.3.40)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.3.40 or 7.5.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (421 amino acids)

>PFR28_04557 Vitamin B12 import ATP-binding protein BtuD (Pseudomonas sp. RS175)
MGYLHVSGLGKAYKQYPNRWSRLFEWLIPFSRQRHQLHWVLQGVDFEIQPGEAVGIVGVN
GAGKSTLLKMITGTTQPTCGHIRLQGRVAALLELGMGFHPDFTGRQNALMAGQLLGMQVE
EIETLMPQIEGFAEIGDAIDQPVRTYSSGMQMRLAFSVATARRPDILIVDEALSVGDAYF
QHKSFERIRSFRKAGTTLLIVSHDRSAIQSICDTAILLENGRMAMRGKPEEVMDYYNAML
AQREGQVVRQEMLANGQVRTISGTGEAGILDVQLLNSQGQPVEVAEVGQPVVLQVRVEVR
QDIERLVLGFLIKDRLGQPMYGINTHRQDQAVTDLLAGEHVTFRFNFDMRLGKGNYSVAL
SLSRLDSHLDRNFEWRDYGLVFHVINNRQEDFVGCSWLQARTSIQRQGLSTAQALPQEEL
P