Protein Info for PFR28_04498 in Pseudomonas sp. RS175

Annotation: Aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00839: aspartate ammonia-lyase
  • amino acids 8 to 468 (461 residues), 687.6 bits, E=4.8e-211
• PF00206: Lyase_1
  • amino acids 17 to 347 (331 residues), 324.2 bits, E=1e-100
• PF10415: FumaraseC_C
  • amino acids 413 to 465 (53 residues), 75.1 bits, 4.7e-25

Best Hits

Swiss-Prot: 99% identical to ASPA_PSEFL: Aspartate ammonia-lyase (aspA) from Pseudomonas fluorescens

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 99% identity to pba:PSEBR_a109)

MetaCyc: 58% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

• L-glutamate degradation II (2/2 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
• L-aspartate degradation II (aerobic) (2/3 steps found)
• L-aspartate degradation III (anaerobic) (2/3 steps found)
• L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.1

Protein Sequence (474 amino acids)

>PFR28_04498 Aspartate ammonia-lyase (Pseudomonas sp. RS175)
MSSAASFRTEKDLLGVLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMVKQAA
ADANRELGQLSEAKHAAISEACARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIANIAL
EAMGHQKGEYQYLHPNNDVNMAQSTNDAYPTAIRLGLLLGHDALLASLESLIQAFAAKGE
EFSHVLKMGRTQLQDAVPMTLGQEFRAFATTLGEDLARLKTLAPELLTEVNLGGTAIGTG
INADPRYQALAVQRLATISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDL
RLLSSGPRTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFQIIGNDLALTMAAEGGQ
LQLNVMEPLIAFKIFDSIRLLQRAMDMLREHCIVGITANEARCRELVEHSIGLVTALNPY
IGYENATRIARVALESGRGVLELVREEGLLDDAMLDDILRPENMIAPRLVPLKA