Protein Info for PFR28_04220 in Pseudomonas sp. RS175

Annotation: Malonyl-S-ACP:biotin-protein carboxyltransferase MADC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 283 transmembrane" amino acids 150 to 175 (26 residues), see Phobius details TIGR03133: biotin-independent malonate decarboxylase, beta subunit" amino acids 11 to 281 (271 residues), 399.2 bits, see alignment E=4.3e-124 PF01039: Carboxyl_trans" amino acids 17 to 232 (216 residues), 52.3 bits, see alignment E=1.9e-18

Best Hits

KEGG orthology group: K13932, malonate decarboxylase beta subunit (inferred from 97% identity to pba:PSEBR_a5302)

MetaCyc: 67% identical to malonate decarboxylase malonyl-[acp] decarboxylase component alpha subunit (Klebsiella pneumoniae)
Biotin-independent malonate decarboxylase. [EC: 4.1.1.88]; Malonyl-S-ACP decarboxylase. [EC: 4.1.1.88, 4.1.1.87]

Predicted SEED Role

"Malonate decarboxylase beta subunit" in subsystem Malonate decarboxylase

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.88

Use Curated BLAST to search for 4.1.1.87 or 4.1.1.88

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (283 amino acids)

>PFR28_04220 Malonyl-S-ACP:biotin-protein carboxyltransferase MADC (Pseudomonas sp. RS175)
MSDSAALLNKHSFVELGARQRAKALLDDGTFRELLDPFQRIMSPWLLRQDVVPQSDDGVV
IAKGCLDGLPVVIAAIEGAFQGGSLGEVGGAKIAGALELAAEDNRKGIPTRAVLLLETGG
VRLQEANLGLAAIAEIHSAIVDLRQYQPVVGVVAGSVGCFGGMSIAAGLCSYLLVTQEAR
LGLNGPQVIEQEAGIDEYDSRDRPFIWSLTGGEQRFATGLVDRYVADDVAQIRQQVGQLL
HLGVPAEYRSGQAELFLQRLARLDTEVQIEPAAVRQLYQGERP