Protein Info for PFR28_04217 in Pseudomonas sp. RS175
Annotation: Malonyl CoA-acyl carrier protein transacylase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K13935, malonate decarboxylase epsilon subunit [EC: 2.3.1.39] (inferred from 92% identity to pba:PSEBR_a5300)MetaCyc: 54% identical to [acyl-carrier protein] S-malonyltransferase (Klebsiella pneumoniae)
Biotin-independent malonate decarboxylase. [EC: 4.1.1.88]; 2.3.1.- [EC: 4.1.1.88]
Predicted SEED Role
"Malonyl CoA acyl carrier protein transacylase (EC 2.3.1.39)" in subsystem Malonate decarboxylase (EC 2.3.1.39)
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
- superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
- superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
- fatty acid biosynthesis initiation (type II) (3/3 steps found)
- malonate degradation I (biotin-independent) (3/3 steps found)
- superpathway of fatty acid biosynthesis initiation (4/5 steps found)
- fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
- fatty acid biosynthesis initiation (plant mitochondria) (1/4 steps found)
- mycobactin biosynthesis (3/11 steps found)
- pederin biosynthesis (2/14 steps found)
- bryostatin biosynthesis (2/19 steps found)
- mupirocin biosynthesis (2/26 steps found)
- corallopyronin A biosynthesis (2/30 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.39, 4.1.1.88
Use Curated BLAST to search for 2.3.1.39 or 4.1.1.88
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (306 amino acids)
>PFR28_04217 Malonyl CoA-acyl carrier protein transacylase (Pseudomonas sp. RS175) MSSLLVFPGQGAQRAGMLHGLSPQVLDEASDALGEDVLQLDNAEALQSTRAVQLCLLIAG VAASRRLLEQAPAPDYVAGLSIGAYPAAVVAGALGFEDALRLVSLRGELMQQAFPQGYGM TAIIGLDLAAVEALLARVHSERTPVYLGNINADNQVVIAGSDEAMSIVAREARSQGAAKA CRLAVSVPSHCPLLEAPARALAQAFADVPLKAPSLGYLSGSRARPMANIEALRDDLAFNM CRVVDWRGTVQSAYERGVRLQIELPPGAVLTGLARRVFEQGTVIAFDGARLDTLQALLRE EGRRRP