Protein Info for PFR28_04167 in Pseudomonas sp. RS175

Annotation: NAD/NADP-dependent betaine aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01804: betaine-aldehyde dehydrogenase
  • amino acids 10 to 477 (468 residues), 755.7 bits, E=7.7e-232
• PF00171: Aldedh
  • amino acids 19 to 479 (461 residues), 607.5 bits, E=7.1e-187

Best Hits

Swiss-Prot: 96% identical to BETB_PSEPF: NAD/NADP-dependent betaine aldehyde dehydrogenase (betB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00130, betaine-aldehyde dehydrogenase [EC: 1.2.1.8] (inferred from 96% identity to pfo:Pfl01_5243)

MetaCyc: 76% identical to betaine aldehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Betaine-aldehyde dehydrogenase. [EC: 1.2.1.8]

Predicted SEED Role

"Betaine aldehyde dehydrogenase (EC 1.2.1.8)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.2.1.8)

MetaCyc Pathways

• choline-O-sulfate degradation (3/3 steps found)
• choline degradation I (2/2 steps found)
• glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
• glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
• glycine betaine biosynthesis III (plants) (2/3 steps found)
• β-alanine biosynthesis I (1/2 steps found)
• β-alanine biosynthesis IV (1/2 steps found)
• choline degradation IV (2/4 steps found)
• dimethylsulfoniopropanoate biosynthesis I (Wollastonia) (1/3 steps found)
• dimethylsulfoniopropanoate biosynthesis II (Spartina) (1/4 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.8

Protein Sequence (490 amino acids)

>PFR28_04167 NAD/NADP-dependent betaine aldehyde dehydrogenase (Pseudomonas sp. RS175)
MARFELQKLYIDGAYSDASGDATFEAINPANGEVLAQVQRATKEDVERAVVSAEKGQKVW
AAMTAMQRSRILRRAVDILRERNDELAALETLDTGKAYSETRYVDIVTGADVLEYYAGLV
PAIEGEQIPLRDTSFVYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSEV
TSLTTLKLAEIYTEAGVPPGVFNVLTGSGREVGTWLTEHPRIEKVSFTGGTDTGKKVMAS
ASSSSLKDVTMELGGKSPLIICDDADLDRAADTAMMANFYSSGQVCTNGTRVFVPSHLKA
AFEAKIVERVARIRIGNPEDENTNFGPLVSFAHMESVLGYIEKGKAEGARLLCGGGRLTD
GEFAKGAFVAPTVFTDCTDDMTIVREEIFGPVMSILTYETEEEAIRRANDTDFGLAAGVV
TRDLNRAHRIIHQLEAGICWINAWGESDAKMPVGGYKQSGVGRENGISSLNNFTRIKSVQ
VELGDYASVF