Protein Info for PFR28_04130 in Pseudomonas sp. RS175

Annotation: Formyltetrahydrofolate deformylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00655: formyltetrahydrofolate deformylase
  • amino acids 9 to 285 (277 residues), 299.9 bits, E=1.1e-93
• PF00551: Formyl_trans_N
  • amino acids 91 to 266 (176 residues), 130.4 bits, E=3.4e-42

Best Hits

Swiss-Prot: 52% identical to PURU_CORS1: Formyltetrahydrofolate deformylase (purU) from Corynebacterium sp. (strain P-1)

KEGG orthology group: K01433, formyltetrahydrofolate deformylase [EC: 3.5.1.10] (inferred from 98% identity to pba:PSEBR_a5210)

Predicted SEED Role

"Formyltetrahydrofolate deformylase (EC 3.5.1.10)" in subsystem One-carbon metabolism by tetrahydropterines (EC 3.5.1.10)

MetaCyc Pathways

• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• folate transformations I (9/13 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.10

Use Curated BLAST to search for 3.5.1.10

Protein Sequence (285 amino acids)

>PFR28_04130 Formyltetrahydrofolate deformylase (Pseudomonas sp. RS175)
MSRAPDTWILTADCPSVLGTVDAVTRFLFEQGCYVTEHHSFDDRLSARFFIRVEFRQPDG
FDEQSFRAGLAERAQAFGMVFELTPPNYRPKVVIMVSKADHCLNDLLYRQRIGQLSMDVV
AVVSNHPDLKPLADWHQIPYYHFPLDPNDKPAQERQVWQVIEDTGAELVILARYMQVLSP
ELCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
VVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL