Protein Info for PFR28_04103 in Pseudomonas sp. RS175

Annotation: Biotin synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00433: biotin synthase
  • amino acids 21 to 316 (296 residues), 408.9 bits, E=6e-127
• PF04055: Radical_SAM
  • amino acids 54 to 211 (158 residues), 81.5 bits, E=8.2e-27
• PF06968: BATS
  • amino acids 226 to 317 (92 residues), 105.4 bits, E=1.5e-34

Best Hits

Swiss-Prot: 97% identical to BIOB_PSEF5: Biotin synthase (bioB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01012, biotin synthetase [EC: 2.8.1.6] (inferred from 97% identity to pfl:PFL_5693)

MetaCyc: 68% identical to biotin synthase (Escherichia coli K-12 substr. MG1655)
Biotin synthase. [EC: 2.8.1.6]

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6)" in subsystem Biotin biosynthesis (EC 2.8.1.6)

MetaCyc Pathways

• biotin biosynthesis I (13/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (3/4 steps found)
• biotin biosynthesis II (4/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.6

Protein Sequence (351 amino acids)

>PFR28_04103 Biotin synthase (Pseudomonas sp. RS175)
MSASTTANLRHDWSLAEVKALFVQPFNDLLFQAQTVHRAHFDPNRVQVSTLLSIKTGACP
EDCKYCPQSGHYNTGLDKEKLLEVQKVLEEAARAKAIGSTRFCMGAAWKHPSAKDMPYVL
EMVKGVKAMGLETCMTLGRLDQEQTEALAKAGLDYYNHNLDTSPEFYGNIITTRTYSERL
QTLAYVRESGMKICSGGILGMGESLDDRANLLIQLANLPEHPESVPINMLVKVAGTPLEN
AEDVDPFDFIRMLAVARILMPKSHVRLSAGREAMNEQMQALAFMAGANSIFYGDKLLTTA
NPQADKDMQLFARLGIQPEAREEHADEVHQAAIEQALVEQKSSEQFYNAAV